Sagiroglu, AArabaci, N2024-06-122024-06-1220051082-60681532-2297https://doi.org/10.1081/PB-200041442https://hdl.handle.net/20.500.14551/22138A simple procedure for the extraction of the lipolytic activity from sunflower seed has been developed. Various conditions of extraction have been optimized in order to obtain maximum yield of lipase. A new lipase enzyme was purified by the fractional salt precipitation from the supernatant, dialysis on a cellulose membrane. and gel column chromatography on Sephadex G-75. The lipase was monomeric. with an apparent M, of 22 kDa and a pI of 8, with the electrophoretic analysis. Kinetics of the enzyme activity versus substrate concentration showed typical lipase behavior. with K-m and V-max values of 1.33 mM and 555 U/mg. All trialycerides were efficiently hydrolyzed by the enzyme, but this showed a preference towards triglycerides of natural mono unsaturated fatty acids. The optimum temperature. pH. and incubation time for lipolytic activity were 50degreesC, 7.5, and 5 min, respectively. The stability of the sunflower lipase was investigated under operational and storage conditions. It was found that this enzyme preserved its lipolytic activity at temperatures between at 35-50degreesC, alkaline pH, and for a period of about four months.en10.1081/PB-200041442info:eu-repo/semantics/closedAccessLipase PurificationCharacterizationSunflower SeedHeliantus Annuus L.Substrate SpecificationRice Bran LipaseFatty-AcidsCastor-OilResolutionGlycerolArticle3513751Q4WOS:0002265912000042-s2.0-1244432879615704496Q3