Sarigul, NeslihanKurultak, IlhanGokceoglu, Arife UsluKorkmaz, Filiz2024-06-122024-06-1220211864-063X1864-0648https://doi.org/10.1002/jbio.202100009https://hdl.handle.net/20.500.14551/18150Urine spectra from 108 healthy volunteers are studied by attenuated total refraction-Fourier transform infrared (ATR-FTIR) spectroscopy. The spectral features are correlated with observable urine components. The variation of spectra within a healthy population is quantified and a library of reference spectra is constructed. Using the band assignments, these spectra are compared with both age-wise and gender-wise. Children show the least intensity variations compared to both adult groups. Young adults show the highest variation, particularly in the 1650 to 1400 cm(-1) and 1200 to 900 cm(-1) regions. These results indicate the importance of the size of the control group in comparative studies utilizing FTIR. Age-wise comparisons reveal that phosphate and sulfate excretion decreases with age, and that the variance of phosphate among individuals is higher with adults. As for gender-wise comparisons, females show a slightly higher citrate content at 1390 cm(-1) regardless of the age and they show a higher variance in the 1200 to 1000 cm(-1) region when compared to men.en10.1002/jbio.202100009info:eu-repo/semantics/closedAccessAdult UrineBand AssignmentChildren UrineFTIRNormal VariationUrine SpectrumTransform Infrared-SpectroscopyHuman Metabolome DatabaseVibrational SpectroscopyCitrate ExcretionPhosphorusSerumHmdbCreatininePhosphateDiagnosisArticle147Q2WOS:0006374537000012-s2.0-8510362453833768707Q1