Ertin, Ismet HandeGunduz, OzgurUlugol, Ahmet2024-06-122024-06-1220151601-5215https://doi.org/10.1017/neu.2014.38https://hdl.handle.net/20.500.14551/18576Background Dipyrone is one of the most commonly used non-opioid analgesic and antipyretic drug. Its anti-nociceptive and hypothermic effects have long been suspected to be centrally mediated. The involvement of the most recently discovered opioid peptide, nociceptin/orphanin FQ (N/OFQ), and its receptor (NOP) in pain transmission is controversial. It appears to be pro-nociceptive when administered supra-spinally, but exerts anti-nociceptive effects when injected spinally or systemically. Objective Investigation of the role of the N/OFQ system in paracetamol-induced anti-nociception and hypothermia led us to determine its role in the anti-nociceptive and hypothermic effects of dipyrone. Material and Methods Hot-plate and tail-flick tests were used to assess nociception, and a rectal thermometer was used to measure rectal temperature in mice. Results Mice injected with dipyrone (150, 300, 600 mg/kg, i.p.) displayed dose-related anti-nociception and hypothermia. The NOP receptor antagonist JTC-801 (3 mg/kg, i.p.), at a dose that exerted no effect when used alone, alleviated dipyrone-induced anti-nociception but did not reverse dipyrone-induced hypothermia. Conclusion We conclude that NOP receptors participate in the anti-nociceptive, but not in the hypothermic, effects of dipyrone.en10.1017/neu.2014.38info:eu-repo/semantics/closedAccessAnti-NociceptionDipyroneHypothermiaJTC-801NOFQEndogenous OpioidsCannabinoid Cb1Chronic PainMiceRatsMetamizolAcetaminophenParacetamolInvolvementInhibitionArticle2714852Q4WOS:0003495318000072-s2.0-8492271844825467825Q2