Özgün, Gülben SayılanÖzgün, ErayTabakçıoğlu, KıymetGökmen, Selma SüerEskiocak, SevgiÇakır, Erol2021-11-202021-11-2020172146-31232146-3131https://app.trdizin.gov.tr/makale/TWpZNE5URTNOdz09https://hdl.handle.net/20.500.14551/5491Background: Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 are antioxidant and anti-atherosclerotic structural high-density lipoprotein proteins that are mainly synthesized by the liver. No study has ever been performed to specifically examine the effects of caffeine on paraoxonase enzymes and on liver apolipoprotein A-1 protein levels. Aims: To investigate the dose-dependent effects of caffeine on liver apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels. Study Design: In vitro experimental study. Methods: HepG2 cells were incubated with 0 (control), 10, 50 and 200 ?M of caffeine for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide assay. ApolipoproteinA-1, paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting. Results: We observed a significant increase on apolipoprotein A-1 and paraoxonase-1 protein levels in the cells incubated with 50 µM of caffeine and a significant increase on paraoxonase-1 protein level in the cells incubated with 200 µM of caffeine. Conclusion: Our study showed that caffeine does not change paraoxonase-3 protein level, but the higher doses used in our study do cause an increase in both apolipoprotein A-1 and paraoxonase-1 protein levels in liver cellsen10.4274/balkanmedj.2016.1217info:eu-repo/semantics/openAccessCerrahiArticle346534539Q3WOS:0004232410000092-s2.0-8503823861729215336Q3268517