Inan, MustafaBasaran, Umit N.Dokmeci, DikmenYalcin, OmerAydogdu, NurettinTuran, Nesrin2024-06-122024-06-1220081440-1681https://doi.org/10.1111/j.1440-1681.2007.04736.xhttps://hdl.handle.net/20.500.14551/20059Testicular ischaemia-reperfusion injury is commonly seen in childhood. Infertility occurs in 25% of patients after unilateral testicular ischaemia. It is has been reported that methylene blue has a positive effect in the reparation of ischaemia-reperfusion injury in different tissues. Therefore, we hypothesized that methylene blue may prevent the hazardous effects of ischaemia-reperfusion injury in testicular tissue after unilateral testicular torsion. Thirty-two prepubertal Wistar-albino rats were divided into four groups. Testicular torsion was created by rotating the right testis 720 degrees in a clockwise direction for 5 h in all groups except for Group C, which was the sham control group. In Group T, bilateral orchiectomy was performed following the torsion period. In Group TD, both testes were removed 5 days after the torsion period. In Group MB, methylene blue (1 mg/kg, i.p.) was administered 40 min before detorsion and once daily over 5 days; then, both testes were harvested. Tissue levels of malondialdehyde (MDA), serum levels of creatine kinase (CK), mean testicular biopsy score (MTBS) and mean seminifer tubule diameter (MSTD) were determined. There was a significant difference in MTBS between Groups T and TD (P < 0.05) in both ipsilateral and contralateral testes. In the contralateral testis, treatment with methylene blue decreased MTBS and MSTD (P < 0.05) and increased MDA levels (P < 0.05). In Group T, mean serum CK concentrations were higher than in any of the other groups (P < 0.05). After 5 h of unilateral testicular torsion and a 5 day reperfusion period, serious tissue damage occurred on both the ipsilateral and contralateral sides. Serum CK concentrations may be an indicator for ischaemia, but not for ischaemia-reperfusion injury. Contrary to our hypothesis, methylene blue increased contralateral testicular damage after unilateral testicular torsion and exacerbated oxidative events.en10.1111/j.1440-1681.2007.04736.xinfo:eu-repo/semantics/closedAccessContralateral TestisCreatine KinaseIschaemia-ReperfusionJohnsen ScoreMalondialdehydeMethylene BlueTesticular TorsionSpermatic Cord TorsionPrepubertal RatsNitric-OxideBlood-FlowTestisAllopurinolAttenuationDamageModelDrugArticle3515054Q3WOS:0002512496000102-s2.0-3664901870218047627Q2