Asav, EnginSagiroglu, AytenSezginturk, Mustafa Kemal2024-06-122024-06-1220161040-03971521-4109https://doi.org/10.1002/elan.201500324https://hdl.handle.net/20.500.14551/19451Calretinin (CAL) is calcium binding protein, and its levels in blood and cerebrospinal fluids are increased, since its expression is increased various cancer types. A novel biosensor system fabricated by immobilization of a specific antibody to CAL, anti-Calretinin (anti-CAL), onto a gold electrode surface via an effective covalent binding method using mercaptohexanol, epichlorohydrin, and ethanolamine was reported for the sensitive, selective, and accurate analysis of CAL. The proposed biosensor showed a linear calibration range between 1 ng/mL and 5 ng/mL. LOD and LOQ values were determined as 0.11 ng/mL and 0.38 ng/mL, respectively. The standard deviation related to the reproducibility of the new biosensor system was calculated as 3.95%. Lastly, in order to state the applicability of the biosensor to early diagnosis of CAL in practice, artificial serum samples spiked with CAL have been analyzed by the proposed biosensor.en10.1002/elan.201500324info:eu-repo/semantics/closedAccessCalretininEpichlorohydrinCancer BiomarkerImmunosensorElectrochemical Impedance SpectroscopySelf-Assembled MonolayersSurface-Plasmon ResonanceHydroxyl-GroupsProteinGoldExpressionActivationImmunohistochemistryAdsorptionDiagnosisArticle282334342Q2WOS:0003701380000112-s2.0-84963657222Q2