Ay, ArzuAlkanli, NevraUstundag, Sedat2024-06-122024-06-1220220163-49841559-0720https://doi.org/10.1007/s12011-021-02828-6https://hdl.handle.net/20.500.14551/19815The aim of this study is to investigate the relationship between IL-18 (- 607 C/A), IL-18 (- 137 G/C), and MMP-2 (- 1306 C/T) gene variations and serum trace element levels in patients diagnosed with CRF. Genotype distributions of IL-18 (- 607 C/A, - 137 G/C) gene variations were determined by polymerase chain reaction (PCR) method. PCR-restriction fragment length polymorphism (RFLP) methods were used to determine the MMP-2 (- 1306 C/T) gene variation genotype distributions. Serum trace element levels were determined by atomic absorption spectrophotometer method. A significant difference was found between the CRF patient and healthy control groups in terms of genotype distributions of IL-18 (- 607 C/A) and MMP-2 (- 1306 C/T) gene variations (p < 0.05). The significant difference was found between the patient and control groups in terms of serum copper and zinc levels and copper/zinc ratio (p < 0.05). The significant difference was detected between patient and control groups in terms of copper and zinc levels and copper/zinc ratio according to IL-18 (- 607 C/A), IL-18 (- 137 G/C), and MMP-2 (- 1306 C/T) gene variations and genotype distributions (p < 0.05). In addition, significant difference was determined in terms of serum copper and zinc levels and copper/zinc ratio according to haplotypes of IL-18 (- 607 C/A), IL-18 (- 137 G/C), and MMP-2 (- 1306 C/T) gene variations between patient and control groups (p < 0.05). In conclusion, evaluation of IL-18 (- 607 C/A, - 137 G/C) and MMP-2 (- 1306 C/T) gene variations and serum trace element levels together is extremely important in terms of obtaining important biomarkers in CRF early diagnosis and progression.en10.1007/s12011-021-02828-6info:eu-repo/semantics/closedAccessChronic Renal FailureIL-18 (-607 CA) Gene VariationIL-18 (-137 GC) Gene VariationMMP-2 (-1306 CT) Gene VariationTrace ElementsPCR-RFLPTrace-ElementsMatrix Metalloproteinase-2PolymorphismsInterleukin-18AssociationPromoterHemodialysisVariantsMetalsArticle200520402052Q2WOS:0006733919000012-s2.0-8512865201934263421Q1