Dinc, ErdemDursun, OzerYilmaz, GulsenKurt, A. HakanAyaz, LokmanVatansever, MustafaOzer, Omer2024-06-122024-06-1220211080-76831557-7732https://doi.org/10.1089/jop.2020.0103https://hdl.handle.net/20.500.14551/18275Purpose: The aim of the present study is to comparatively evaluate the anti-inflammatory and antiapoptotic effects of bone marrow and adipose-derived mesenchymal stem cells (MSCs) applied subconjunctivally after alkaline corneal burn. Methods: Thirty-two rats were divided into 4 groups and included in the study (n = 8). While no intervention was made in the control group, a chemical burn was created by applying 4 mu L of NaOH soaked in 6 mm filter paper to the right eye of each subject in the other groups under general anesthesia. While only subconjunctival 0.1 mL phosphate-buffered saline (PBS) was injected to in the group 1, 2 x 10(6) adipose or bone marrow-derived MSC in 0.1 mL PBS was applied subconjunctivally to the subjects in the remaining groups (Group 2 and 3, respectively). Tissue samples were collected for histological analysis on the third day after the burn. Tissue samples were evaluated light microscopically and immunohistochemically stained for interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), caspase-3 (Cas-3), and CD68. Results: The IL-1 beta and TNF-alpha staining scores and the number of CD68- and Cas-3-positive stained cells were significantly lower in the groups given bone marrow and adipose-derived MSC compared to the alkaline burn group (P < 0.0001, for all parameters). Epithelial IL-1 beta and TNF-alpha staining scores were significantly lower in the bone marrow-derived MSC group compared to the adipose-derived MSC group (P < 0.0001, for all parameters). Conclusions: The presented study shows that both bone-marrow and adipose-derived MSCs support wound healing in the corneal tissue and strongly suppress the inflammation occured in the tissue.en10.1089/jop.2020.0103info:eu-repo/semantics/closedAccessAlkaline BurnApoptosisChemical Corneal BurnInflammationMesenchymal Stem CellWound HealingSurfaceReconstructionArticle3712434Q2WOS:0005964003000012-s2.0-8509973265933275515Q2