Huseyin, SerhatGuclu, OrkutYuksel, VolkanErkul, Gulen Sezer AlptekinCan, NurayTuran, Fatma NesrinCanbaz, Suat2024-06-122024-06-1220170102-76381678-9741https://doi.org/10.21470/1678-9741-2016-0081https://hdl.handle.net/20.500.14551/23599Objective: Ischemia-reperfusion injury after acute ischemia treatment is a serious condition with high mortality and morbidity. Ischemia-reperfusion injury may result in organ failure particularly in kidney, lung, liver, and heart. In our study, we investigated the effects of papaverine and vitamin C on ischemia-reperfusion injury developed in the rat liver after occlusion-reperfusion of rat aorta. Methods: 32 Sprague-Dawley female rats were randomized into four groups (n=8). Ischemia was induced with infrarenal aortic cross-clamping for 60 minutes; then the clamp was removed and reperfusion was allowed for 120 minutes. While the control group and the ischemia-reperfusion group did not receive any supplementary agent, two other groups received vitamin C and papaverine hydrochloride (papaverine HCL). Liver tissues were evaluated under the light microscope. Histopathological examination was assessed by Suzuki's criteria and results were compared between groups. Results: In ischemia-reperfusion group, severe congestion, severe cytoplasmic vacuolization, and parenchymal necrosis over 60% (score 4) were observed. In vitamin C group, mild congestion, mild cytoplasmic vacuolization and parenchymal necrosis below 30% (score 2) were found. In papaverine group, moderate congestion, moderate cytoplasmic vacuolization and parenchymal necrosis below 60% (score 3) were observed. Conclusion: An ischemia of 60 minutes induced on lower extremities causes damaging effects on hepatic tissue. Vitamin C and papaverine are helpful in reducing liver injury after acute ischemia reperfusion and may partially avoid related negative conditions.en10.21470/1678-9741-2016-0081info:eu-repo/semantics/openAccessReperfusion InjuryLiver DiseasesPapaverineAscorbic AcidRatsModels, AnimalIschemia-Reperfusion InjuryLung InjuryRatPathophysiologyMelatoninArticle323197201Q4WOS:0004183572000102-s2.0-8502771539928832798Q3