Demirkiran O.Ahmad Mesaik M.Beynek H.Abbaskhan A.Iqbal Choudhary M.2024-06-122024-06-1220131307-6167https://hdl.handle.net/20.500.14551/17572The MeOH extract of aerial parts of H. monbretii Spach. have yielded quercetin (1), kaempferol (2), quercitrin (3), hyperin (4), myricitrin (5), rutin (6), I 3 II 8? biapigenin (7), methyl chlorogenate (8), 5-O-pcoumaroylquinic acid (9), and chlorogenic acid (5-O-caffeoyl quinic acid) (10), mangiferin (11), and shikimic acid (12). Three compounds 1, 2 and 4 were found to have an immunomodulating inhibitory activity on the oxidative burst response of human neutrophils, as well on mouse peritoneal macrophages. Effects of compounds on whole blood phagocytes activity was found to be with IC50 ranging between 8.0-12.2 ?g/mL. Only compounds 1, and 2 were found to interfere with T-cell proliferation, and IL-2 release in PHA activated peripheral blood mononuclear cells system. To the best of our knowledge, this is the first report of phytochemical and biological activity study on H. montbretii Spach.eninfo:eu-repo/semantics/closedAccessHypericum Monbretii Spach.; Il-2 Production; Immunomodulating Inhibitory Activity; Phenolics; Reactive Oxygen Species; T-Cell Proliferation5 O 4 Coumaroylquinic Acid; 8'' Biapigenin; Chlorogenic Acid; Hypericum Montbretii Extract; Hyperin; Immunosuppressive Agent; Interleukin 2; Kaempferol; Mangiferin; Methanol; Methyl Chlorogenate; Myricetin 3 Rhamnoside; Phenol Derivative; Phytohemagglutinin; Plant Extract; Quercetin; Quercitrin; Rutoside; Shikimic Acid; Unclassified Drug; Animal Cell; Article; Cell Activation; Controlled Study; Cytokine Production; Cytokine Release; Drug Isolation; Drug Structure; Growth Inhibition; Human; Human Cell; Hypericum; Hypericum Montbretii; Ic 50; Immunomodulation; Incubation Time; Lymphocyte Proliferation; Mouse; Neutrophil; Nonhuman; Peripheral Blood Mononuclear Cell; Peritoneum Macrophage; Phagocyte; Phytochemistry; Respiratory Burst; T Lymphocyte; HypericumArticle732102192-s2.0-84878426155Q3