Kok, SuleymanAtalay, SertacSavasci, MustafaEken, Hasan Semih2024-06-122024-06-1220131300-6045https://doi.org/10.9775/kvfd.2012.7470https://search.trdizin.gov.tr/yayin/detay/140394https://hdl.handle.net/20.500.14551/23060Calpastatin (CAST) is a specific inhibitor of calcium-dependent neutral protease mu-calpain found in mammalian tissues. The genetic variants in the bovine CAST gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The animal material of the study consisted of 132 bulls and heifers in Turkish Grey steppe cattle and Turkish Grey steppe x Brown Swiss crossbreds. C and G alleles are characterized by CAST/RsaI polymorphism were detected in the sample of animals were studied. Allele frequency C was significantly frequent in the crossbred group compared to the pure Turkish Grey steppe animals (P<0.05). In the total samples of animals, the average allele frequency C was 56.1%. Genotypic frequencies were estimated as 0.257, 0.499 and 0.243 in the purebred Turkish Grey steppe, and 0.388, 0.470 and 0.142 in the Turkish Grey steppe x Brown Swiss crossbred population for genotypes CC, CG and GG, respectively. As a result, genotypic distributions were equilibrium in both pure and crossbred examples of Turkish Grey steppe cattle.en10.9775/kvfd.2012.7470info:eu-repo/semantics/closedAccessPCR-RFLPGrey Steppe CattleCalpastatin (CAST)Genetic MarkerPolymorphismSingle Nucleotide PolymorphismsBeef-CattleM. LongissimusAssociationTendernessTraitsCarcassCapn1DnaArticle192203206Q4WOS:0003217484000042-s2.0-84872784401Q3140394