Eskiocak, SevgiÖzgün, ErayÖzgün, Gülben SayılanTabakçıoğlu, KıymetGökmen, Selma Süer2024-06-122024-06-1220192564-6567https://doi.org/10.25000/acem.623975https://search.trdizin.gov.tr/yayin/detay/319440https://hdl.handle.net/20.500.14551/12924Aim: Palmitate is one of the most abundant fatty acid in both liver of healthy individuals and in patients with non-alcoholic fatty liver disease. Palmitate-induced steatosis in HepG2 cells is an in vitro non-alcoholic fatty liver disease model to investigate acute harmful effects of fat overaccumulation in the liver. Non-alcoholic fatty liver disease is strongly associated with atherosclerosis. Paraoxonase-1 and paraoxonase-3 are anti-atherosclerotic enzymes which are bound to high density lipoprotein in circulation and they are primarily synthesized by liver. There is no study that investigated the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes. The aim of present study was to investigate the effect of palmitate-induced steatosis on paraoxonase-1 and paraoxonase-3 enzymes in HepG2 cells. Methods: To induce steatosis, cells were incubated with 0.4, 0.7 and 1 mM palmitate for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Cells were stained with oil red O and triglyceride levels were measured. Paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting, their mRNA expression were measured by quantitative PCR and arylesterase activity was measured spectrophotometrically. Results: All palmitate concentrations caused a significant increase on paraoxonase-1 mRNA levels. Palmitate concentrations did not cause a significant change on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA levels and arylesterase activities. Conclusion: Our study showed that palmitate-induced steatosis up-regulates paraoxonase-1 mRNA, has no effect on paraoxonase-1 and paraoxonase-3 protein levels, paraoxonase-3 mRNA and arylesterase activity in HepG2 cells.en10.25000/acem.623975info:eu-repo/semantics/openAccessArticle43142147319440