Say, RidvanGultekin, AytacOzcan, Ayca AtilirDenizli, AdilErsoz, Arzu2024-06-122024-06-1220090003-26701873-4324https://doi.org/10.1016/j.aca.2009.03.022https://hdl.handle.net/20.500.14551/22524The routine measurement of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in biological samples is a difficult analytical problem due to the low levels of the analyte and complex matrix. A new 8-OHdG imprinted quartz crystal microbalance (QCM) sensor has been developed for selective determination of 8-OHdG in serum samples. To fulfil the desired results, we have used methacryloyl aminoantipyrine-Fe(III) [MAAP-Fe(III)] and methacryloyl histidine-Pt(II) [MAH-Pt(II)] as metal-chelating monomers via double metal coordination-chelation interactions for the preparation of additional selective molecular imprinted polymers (MIP). The study includes the measurement of binding interaction of 8-OHdG imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM chip. The obtained results have showed that the application of double metal-chelate monomer systems has been more effective than single metal-chelate monomer systems. In this study, the detection limit and the linear working range were found to be 0.0075 and 0.0100-3.5 mu M, respectively. The affinity constant (K-affinity) was found to be 1.54 x 10(5) M-1 for 8-OHdG using MAH-Pt-8-OHdG-MAAP-Fe based thin film. Also, selectivity of prepared QCM sensor was found as being very high in the presence of competitive species. At the last step of this procedure, 8-OHdG level in blood serum which belongs to a intestinal cancer patient was determined by the prepared QCM sensor. (c) 2009 Elsevier B.V. All rights reserved.en10.1016/j.aca.2009.03.022info:eu-repo/semantics/closedAccessMolecular Imprinted PolymersQuartz Crystal Microbalance8-Hydroxy-2 '-DeoxyguanosineMetal-Chelate InteractionOxidative Dna-DamageSubstrate-Selective PolymersLiquid-Chromatography8-HydroxydeoxyguanosineRecognitionImmunosensorRemovalMarkerUrinePreparation of new molecularly imprinted quartz crystal microbalance hybride sensor system for 8-hydroxy-2?-deoxyguanosine determinationArticle6401-28286Q1WOS:0002657635000122-s2.0-6404908428819362624Q1