Hasancebi, Semra2024-06-122024-06-122017978-1-4939-7249-4978-1-4939-7248-71064-37451940-6029https://doi.org/10.1007/978-1-4939-7249-4_9https://hdl.handle.net/20.500.14551/23391Wheat-rust interactions are extremely complex biological processes which are accompanied with the defense/attack responses to survive and overcome pathogen attack or plant defense. Understanding of molecular mechanism of these interactions is a promising way to develop sustainable combat. Therefore, many studies have been performed to reveal the active host and pathogen-derived genes and their products during the infection or defense using different approaches for many decades. Particularly proteomics technology and proteome profiling which is a large scale analysis of a protein mixture to reveal differently expressed proteins under a certain conditions has become a very important tool for providing real insights into the extremely complex interactions. Moreover, this type of research has the potential to explore target proteins/genes such as effectors that can be used in disease management strategies. Hence, in this chapter we describe the proteome profiling protocols by using 2D-LC system.en10.1007/978-1-4939-7249-4_9info:eu-repo/semantics/closedAccessProteomicsProteome ProfilingPlant-Pathogen InteractionDefense2D-LCPF2DLeaf Protein ExtractionWheatStripe Rust2-Dimensional Liquid-ChromatographyFractionationBook Chapter165999113N/AWOS:0004313437000102-s2.0-8502897282028856644Q3