Bese, N. S.Umay, C.Serdengecti, S.Kepil, N.Sut, N.Altug, T.Ober, A.2024-06-122024-06-1220101357-05601559-131Xhttps://doi.org/10.1007/s12032-009-9395-5https://hdl.handle.net/20.500.14551/243916th European Breast Cancer Conference -- APR 15-19, 2008 -- Berlin, GERMANYThere are no data regarding the late toxicity of trastuzumab (T) administration with radiotherapy (RT). In this experimental study, we aimed to asses if concurrent or sequential administration of T has any impact for the development of radiation-induced pulmonary fibrosis in rats. Fifty-four female Wistar-albino rats were divided into 6 groups. First group of rats (Group 1; concurrent T) had irradiation to whole thoracic region concurrently with T. Second group (Group 2: sequential T-RT) received thoracic irradiation, 1 week after T. Third group (Group 3: sequential RT-T) had thoracic irradiation first and they had T injection 1 week after RT. Fourth group (Group 4: T only) had only T application. Fifth group (Group 5: RT) had only RT. The last group (Group 6: sham) of rats were observed without any application. A single dose of 12 Gy was given to both lungs with an anterior field at 2 cm depth. T dose which was equivalent to 6 mg/kg adult dose was calculated for each rat, and injected by the tail vein. As an end point the extent of pulmonary fibrosis for each field was graded on a scale from 0 (normal lung or minimal fibrous thickening) to 4 (total fibrous obliteration of the field) at histopathological examination. The mean value of fibrosis scores were 1.44, 1.77, 1.75 and 1.62 for Group 1, 2, 3 and 5, respectively, without any statistically significant differences among them (P > 0.05). The mean value of fibrosis scores for Group 4 and 6 were 0.25 and 0.33, respectively (P > 0.05). When the mean value of fibrosis scores of the groups which had RT with or without T, compared with the observation and the T only groups, the difference was significant (P < 0.05) (one-way ANOVA and Tukey HSD post hoc tests) As a conclusion: addition of T to thoracic irradiation either sequentially or concomitantly did not increase radiation-induced pulmonary fibrosis in rats.en10.1007/s12032-009-9395-5info:eu-repo/semantics/closedAccessTrastuzumabRadiation TherapyLate ToxicityHer2-Positive Breast-CancerConcurrent TrastuzumabAdjuvant ChemotherapyMonoclonal-AntibodyFollow-UpRadiotherapyPlusIrradiationToxicityConference Object27414151419Q3WOS:0002845478000612-s2.0-7995177056520041318Q2