Alkanli, NevraAy, Arzu2024-06-122024-06-1220232980-1478https://doi.org/10.5152/tud.2023.22185https://search.trdizin.gov.tr/yayin/detay/1187870https://hdl.handle.net/20.500.14551/24521Objective: Bladder cancer is a complex malignancy and has been associated with high morbidity. Since susceptibility to bladder cancer development differs between individuals, determining the roles of MTHFR and MMP-2 gene variations associated with this cancer is important for analyzing differences in individual susceptibility. In this study, we aimed to investigate the role of MTHFR and MMP-2 gene variations in the development of bladder cancer in the Thrace region of Turkey. Materials and methods: One hundred seventy-nine blood samples were collected, including 98 patients with bladder cancer and 81 healthy controls. DNA extraction was carried out with blood samples. Polymerase chain reaction-restriction fragment length polymorphism was applied to detect MTHFR C677T (rs 1801133), MTHFR A1298C (rs 1801131), and MMP-2 (-1306C>T) (rs 243865) gene variants. Results: For the MTHFR A1298C gene variation, CC genotype was the genetic risk factor (P =.0001), while AC genotype was the protective factor (P <.0001) in the development of bladder cancer. For the MMP-2 (-1306C>T) gene variation, TT genotype (P <.0001) and T allele (P =.0006) were genetic risk factors, while AC genotype (P =.0009) was the protective factor in the development of bladder cancer. For C677T/A1298C gene variations, CC-CC combined genotype was the genetic risk factor (P =.009), while CT-AC and CC-AC combined genotypes were potential protective biomarkers (P =.013 and P <.001, respectively). Conclusion: In our study, TT genotype and T allele were determined as genetic risk factors for MMP-2 (-1306C>T) gene variation. For C677T/A1298C gene variations, CCCC combined genotype was detected as the genetic risk factor in the development of bladder cancer.en10.5152/tud.2023.22185info:eu-repo/semantics/openAccessUrinary Bladder NeoplasmsMethylenetetrahydrofolate ReductasePolymorphismMatrix MetalloproteinasesPolymerase Chain ReactionRestriction Fragment Length PolymorphismGene PolymorphismsRiskSusceptibilityDeficiencyDnaArticle4913339N/AWOS:0010042599000072-s2.0-8515099253437877836N/A1187870