Donmez, AyhanOzsan, FatmaArik, BaharOzkayin, NeseCagirgan, SeckinMir, SevgiVural, Filiz2024-06-122024-06-1220131473-0502https://doi.org/10.1016/j.transci.2013.01.005https://hdl.handle.net/20.500.14551/19622There is no detailed information about the clearance time of infused hematopoietic stem cell (HSC) from the blood circulation in humans. In this prospective study, peripheral blood CD34+ cell counts were detected during the 4 days period following autologous HSC transplantation in 20 patients by means of flow cytometry. The median CD34+ cells were at the highest level in the first hour and decreased below pre-infusion values on the first day after HSC infusion. By nonparametric analysis, positive correlation was found between CD34+ cell levels at the first hour and the post-thaw CD34+ cell dose (r = 0.57, p = 0.01). An inverse correlation was determined between CD34+ cell levels at the first hour and neutrophil engraftment (r = -0.54, p = 0.01). Compared with the patients having CD34+ cell count of >= 2 mu L-1 in the first hour following HSC infusion, the patients having CD34+ cell count of <2 mu L-1 had delayed both neutrophil (20 vs. 12, p = 0.008) and platelet (47 vs. 11, p = 0.01) engraftments. Our results indicated that infused HSCs were removed from the blood circulation within 1 day. In addition, CD34+ cell levels at the first hour may be used as an important indicator to predict the delay of neutrophil and platelet engraftments. (c) 2013 Elsevier Ltd. All rights reserved.en10.1016/j.transci.2013.01.005info:eu-repo/semantics/closedAccessCD34+Cell LevelsEngraftmentHomingStem Cell TransplantationProgenitor CellsTraffickingEngraftmentArticle482235239Q4WOS:0003195383000462-s2.0-8487763266723428427Q3