Benlier, ErolEskiocak, SevgiPuyan, Fulya OzKandulu, HuseyinUnal, YasinTop, HusamettinAygit, Ahmet Cemal2024-06-122024-06-1220110305-41791879-1409https://doi.org/10.1016/j.burns.2011.04.008https://hdl.handle.net/20.500.14551/207276th Congress of the Balkan-Association-of-Plastic-Reconstructive-and-Aesthetic-Surgery -- JUN 04-07, 2009 -- Ohrid, MACEDONIABackground: Electrical injuries induce progressive tissue loss caused by free oxygen radicals released from neutrophil aggregates. Fucoidin, a potent inhibitor of L-selectin function, reduces the aggregation of neutrophils. The aim of this study was to evaluate the effect of fucoidin on tissue damage in rat electrical burn injury model. Methods: Forty-two male Wistar albino rats (250-300 g) were divided into 3 groups (Group A (n = 6), control group without electrical burn injury; Groups B (n = 18) and C (n = 18), electrical burn injury groups without and with fucoidin therapy, respectively). Three separate analyses were performed at different time points on 6 out of 18 mice from Group B and C at each time point. Biochemistry (myeloperoxidase and malondialdehyde levels) and histopathology (number of neutrophils) of the skin and muscle biopsies at 1st hour; tissue edema (ratio of wet weight/dry weight of extremities) at 24th hour; and necrotic areas at 7th day after electrical injury were evaluated. The electrical burn was induced by exposing rats to 220 V AC between their left upper extremity and right lower extremity for 10 s. Fucoidin was administered as 25 mg/kg intravenous bolus injection at 15 mm after electrical burn injury. Results: Myeloperoxidase and malondialdehyde levels, number of neutrophils, tissue edema, and necrotic area were significantly less in fucoidin-applied rats than the group without fucoidin therapy. Conclusions: Fucoidin inhibits tissue damage induced by electrical burn injury in rats by reducing necrotic area, edema and number of neutrophils. (C) 2011 Elsevier Ltd and ISBI. All rights reserved.en10.1016/j.burns.2011.04.008info:eu-repo/semantics/closedAccessElectric BurnFucoidinNeutrophilMyeloperoxidaseMalonyldialdehydeIschemia-Reperfusion InjuryThermal-InjuryPolysaccharide FucoidinLipid-PeroxidationMeningitisManagementAdhesionTraumaSkinConference Object37712161221Q2WOS:0002960756000192-s2.0-8005296104721641115Q1