Nemmar, AbderrahinnKaraca, TuranBeegam, SumayaYuvaraju, PriyaYasin, JavedHamadi, Naserddine KamelAli, Badreldin H.2024-06-122024-06-1220161015-89871421-9778https://doi.org/10.1159/000443109https://hdl.handle.net/20.500.14551/18225Background/Aims: Epidemiological evidence indicates that patients with chronic kidney diseases have increased susceptibility to adverse outcomes related to long-term exposure to particulate air pollution. However, mechanisms underlying these effects are not fully understood. Methods: Presently, we assessed the effect of prolonged exposure to diesel exhaust particles (DEP) on chronic renal failure induced by adenine (0.25% w/w in feed for 4 weeks), which is known to involve inflammation and oxidative stress. DEP (0.5m/kg) was intratracheally (i.t.) instilled every 4th day for 4 weeks (7 i.t. instillation). Four days following the last exposure to either DEP or saline (control), various renal endpoints were measured. Results: While body weight was decreased, kidney weight increased in DEP+adenine versus saline+adenine or DEP. Water intake, urine volume, relative kidney weight were significantly increased in adenine+DEP versus DEP and adenine+saline versus saline. Plasma creatinine and urea increased and creatinine clearance decreased in adenine+DEP versus DEP and adenine+saline versus saline. Tumor necrosis factor alpha, lipid peroxidation and reactive oxygen species were significantly increased in adenine+DEP compared with either DEP or adenine+saline. The antioxidant calase was significantly decreased in adenine+DEP compared with either adenine+saline or DEP Notably, renal DNA damage was significantly potentiated in adenine+DEP compared with either adenine+saline or DEP Similarly, systolic blood pressure was increased in adenine+DEP versus adenine+saline or DEP, and in DEP versus saline. Histological evaluation revealed more collagen deposition, higher number of necrotic cell counts and dilated tubules, cast formation and collapsing glomeruli in adenine+DEP versus adenine+saline or DEP. Conclusion: Prolonged pulmonary exposure to diesel exhaust particles worsen renal oxidative stress, inflammation and DNA damage in mice with adenine-induced chronic renal failure. Our data provide biological plausibility that air pollution aggravates chronic renal failure. (C) 2016 The Author(s) Published by S. Karger AG, Baselen10.1159/000443109info:eu-repo/semantics/openAccessAir PollutionDiesel Exhaust ParticlesAdenineChronic Kidney FailureMiceDNA DamageOxidative StressCisplatin-Induced NephrotoxicityParticulate MatterAccelerates CoagulationCardiovascular-DiseaseChronic KidneyAir-PollutionGum AcaciaRatsToxicityNoseArticle38517031713Q1WOS:0003757878000042-s2.0-8496627024827160713Q2