Distribution of insoluble polysaccharides, neutral lipids, and proteins in the developing anthers of Campsis radicans (L.) Seem. (Bignoniaceae)

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dc.authoridKonyar, Sevil Tütüncü/0000-0002-0737-7818
dc.authorwosidKonyar, Sevil Tütüncü/AAT-6279-2020
dc.authorwosidKonyar, Sevil Tütüncü/F-6708-2013
dc.contributor.authorKonyar, Sevil Tutuncu
dc.contributor.authorDane, Feruzan
dc.contributor.authorTutuncu, Serpil
dc.date.accessioned2024-06-12T10:54:28Z
dc.date.available2024-06-12T10:54:28Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, distribution of polysaccharides, lipids, and proteins in the developing anthers of Campsis radicans (L.) Seem. was examined from sporogenous cell stage to mature pollen, using cytochemical methods. To detect the distribution and dynamic changes of insoluble polysaccharides, lipid bodies, and proteins in the anthers through progressive developmental stages, semi-thin sections of anthers at different developmental stages were stained with periodic-acid-Schiff (PAS) reagent, Sudan black B, and Coomassie brilliant blue, respectively, and examined under light microscope. Ultrastructural observations with TEM were also carried out to determine the storage form of starch in the connective tissue, and storage form of lipids in the tapetal cells. In sporogenous cell stage, anther wall contains numerous insoluble polysaccharides. However, from the sporogenous cell stage to the vacuolated microspore stage, the amount of insoluble polysaccharides in the anther wall decreases gradually. At bicellular pollen stage, tapetum degenerates completely and polysaccharides are not seen in the anther wall. Lipid bodies are observed in the cytoplasm of both middle layer and tapetal cells at tetrad stage, whereas they disappear in the vacuolated microspore stage. Compared with polysaccharides, proteins are limited in the anther wall at early stages of development. During pollen development, polysaccharides, proteins, and lipid bodies are scarce in the cytoplasm of sporogenous cells, but their amount increases at premeiotic stage. From tetrad stage to bicellular pollen stage, microspore cytoplasm contains variable amount of insoluble polysaccharide grains, lipid and protein bodies. At bicellular pollen stage, plentiful amount of starch granules are stored in the cytoplasm of the pollen grains. Proteins and lipid bodies are also present in the cytoplasm.en_US
dc.description.sponsorshipTrakya Universityen_US
dc.description.sponsorshipWe would like to thank the Scientific Research Fund of Trakya University, which financially supported this study.en_US
dc.identifier.doi10.1007/s00606-013-0758-1
dc.identifier.endpage760en_US
dc.identifier.issn0378-2697
dc.identifier.issn2199-6881
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84874947501en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage743en_US
dc.identifier.urihttps://doi.org/10.1007/s00606-013-0758-1
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19066
dc.identifier.volume299en_US
dc.identifier.wosWOS:000316262300005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringer Wienen_US
dc.relation.ispartofPlant Systematics And Evolutionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBignoniaceaeen_US
dc.subjectCampsisen_US
dc.subjectAnther Histochemistryen_US
dc.subjectPolysaccharideen_US
dc.subjectLipid Distributionen_US
dc.subjectAnther Wallen_US
dc.subjectMale Gametophyteen_US
dc.subjectPollen Ontogenyen_US
dc.subjectMale-Sterilityen_US
dc.subjectStarchen_US
dc.subjectTapetumen_US
dc.subjectLiliumen_US
dc.subjectWallen_US
dc.subjectLocalizationen_US
dc.subjectGrainsen_US
dc.titleDistribution of insoluble polysaccharides, neutral lipids, and proteins in the developing anthers of Campsis radicans (L.) Seem. (Bignoniaceae)en_US
dc.typeArticleen_US

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