Characterization of natural orchardgrass (Dactylis glomerata L.) populations of the Thrace Region of Turkey based on ploidy and DNA polymorphisms

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dc.authoridKhadka, Deepak/0000-0001-6649-3328;
dc.authorwosidKhadka, Deepak/J-3414-2015
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.contributor.authorTuna, M
dc.contributor.authorKhadka, DK
dc.contributor.authorShrestha, MK
dc.contributor.authorArumuganathan, K
dc.contributor.authorGolan-Goldhirsh, A
dc.date.accessioned2024-06-12T10:59:08Z
dc.date.available2024-06-12T10:59:08Z
dc.date.issued2004
dc.departmentTrakya Üniversitesien_US
dc.description.abstractDetermining the ploidy and genetic diversity of a germplasm is necessary before initiating breeding or genetic studies. This study was conducted to characterize the ploidy level of 57 natural populations of orchardgrass (Dactylis glomerata L.) collected from the ranges of Thrace region of Turkey and the diversity among populations based on RAPD (Random Amplified Polymorphic DNA) markers. Flow cytometry was used to determine nuclear DNA content (pg 2C(-1) = DNA content of a diploid somatic nucleus) of 6 plants for each population. Nuclear DNA contents were correlated to ploidy level with root tip chromosome counts on selected plants. On the basis of this study, mean nuclear DNA content of orchardgrass was determined as 9.5 pg +/- 0.33 (with 95% confidence interval) while all the plants used in chromosome counting were determined to be tetraploid, with 2n=28 mitotic chromosomes, suggesting that diploid orchardgrass plants are likely very rare or absent in ranges of Thrace region of Turkey. In the RAPD assay, over 40 polymorphic fragments were generated which allowed some populations to be distinguished from the rest by unique markers. A cluster analysis was performed using Nei's (1972) genetic distance index with an unweighted pair group method with arithmetic mean (UPGMA). The cluster analysis indicated that there is a high level of gene flow among natural orchardgrass populations and therefore genes distributed quite homogeneously through out the region. The results of this study can be useful in the development of Dactylis germ plasm collection strategies in Thrace region for breeding purpose.en_US
dc.identifier.doi10.1023/B:EUPH.0000009537.08697.4e
dc.identifier.endpage46en_US
dc.identifier.issn0014-2336
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-0742302393en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage39en_US
dc.identifier.urihttps://doi.org/10.1023/B:EUPH.0000009537.08697.4e
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20331
dc.identifier.volume135en_US
dc.identifier.wosWOS:000187426700005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherKluwer Academic Publen_US
dc.relation.ispartofEuphyticaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDactylis Glomerataen_US
dc.subjectGenetic Diversityen_US
dc.subjectNuclear DNA Contenten_US
dc.subjectOrchardgrassen_US
dc.subjectPloidyen_US
dc.subjectRandom Amplified Polymorphic DNAen_US
dc.subjectRapd Markersen_US
dc.subjectGenetic-Relationshipsen_US
dc.subjectGermplasm Accessionsen_US
dc.subjectArbitrary Primersen_US
dc.subjectFlow-Cytometryen_US
dc.subjectCultivarsen_US
dc.subjectEvolutionen_US
dc.subjectComplexen_US
dc.subjectCollectionsen_US
dc.subjectVariabilityen_US
dc.titleCharacterization of natural orchardgrass (Dactylis glomerata L.) populations of the Thrace Region of Turkey based on ploidy and DNA polymorphismsen_US
dc.typeArticleen_US

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