Heat stress decreases testicular germ cell proliferation and increases apoptosis in short term: an immunohistochemical and ultrastructural study

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dc.authorwosidAktas, Cevat/D-8468-2011
dc.contributor.authorKanter, Mehmet
dc.contributor.authorAktas, Cevat
dc.contributor.authorErboga, Mustafa
dc.date.accessioned2024-06-12T11:12:08Z
dc.date.available2024-06-12T11:12:08Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractScrotal hyperthermia has been known as a cause of male infertility but the exact mechanism leading to impaired spermatogenesis is unknown. This work was aimed to investigate the role of scrotal hyperthermia on cell proliferation and apoptosis in testes. The rats were randomly allotted into one of the four experimental groups: A (control), B (1 day after scrotal hyperthermia), C (14 days after scrotal hyperthermia), and D (35 days after scrotal hyperthermia); each group comprised 7 animals. Scrotal hyperthermia was carried out in a thermostatically controlled water bath at 43 degrees C for 30 min once daily for 6 consecutive days. Control rats were treated in the same way, except the testes were immersed in a water bath maintained at 22 degrees C. Hyperthermia-exposed rats were killed under 50 mg/kg ketamine anaesthesia and tissue samples were obtained for biochemical and histopathological investigations. Hyperthermia treatment significantly decreased the testicular antioxidant system, including decreases in the glutathione level, superoxide dismutase, and glutathione peroxidase activities. Moreover, exposure to hyperthermia resulted in lipid peroxidation increase in testes. Our data indicate a significant reduction in the expression of proliferating cell nuclear antigen and an enhancement in the activity of terminal deoxynucleotidyl transferase dUTP nick end labelling after scrotal hyperthermia. In scrotal hyperthermia, the mitochondrial degeneration, dilatation of smooth endoplasmic reticulum, and enlarged intercellular spaces were observed in both Sertoli and spermatid cells. Scrotal hyperthermia is one of the major factors that impair spermatogenesis in testis. This heat stress is shown to be closely associated with oxidative stress, followed by apoptosis of germ cells.en_US
dc.identifier.doi10.1177/0748233711425082
dc.identifier.endpage113en_US
dc.identifier.issn0748-2337
dc.identifier.issn1477-0393
dc.identifier.issue2en_US
dc.identifier.pmid22082826en_US
dc.identifier.scopus2-s2.0-84874440517en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage99en_US
dc.identifier.urihttps://doi.org/10.1177/0748233711425082
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23058
dc.identifier.volume29en_US
dc.identifier.wosWOS:000315761000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Incen_US
dc.relation.ispartofToxicology And Industrial Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPCNAen_US
dc.subjectTUNELen_US
dc.subjectUltrastructureen_US
dc.subjectScrotal Hyperthermiaen_US
dc.subjectRaten_US
dc.subjectSingle Exposureen_US
dc.subjectGnrh Agonisten_US
dc.subjectHyperthermiaen_US
dc.subjectRatsen_US
dc.subjectSpermatogenesisen_US
dc.subjectSuppressionen_US
dc.subjectGlutathioneen_US
dc.subjectMechanismsen_US
dc.subjectEpitheliumen_US
dc.subjectInductionen_US
dc.titleHeat stress decreases testicular germ cell proliferation and increases apoptosis in short term: an immunohistochemical and ultrastructural studyen_US
dc.typeArticleen_US

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