Endothelial Nitric Oxide Synthase Immunreactivity and the Ultrastructure of Endothelial Cells of Umbilical Artery in Normal and Preeclamptic Pregnancies

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dc.contributor.authorKanter, Mehmet
dc.contributor.authorGurbuz, Hulya
dc.contributor.authorOkman, Tulay Kilic
dc.date.accessioned2024-06-12T11:16:56Z
dc.date.available2024-06-12T11:16:56Z
dc.date.issued2010
dc.departmentTrakya Üniversitesien_US
dc.description.abstractOur objective was to investigate the endothelial nitric oxide synthase (eNOS) immuno-reactivity and the ultrastructure of endothelial cells of a human umbilical artery in both normal and preeclamptic pregnancies. The umbilical cords from normal and preeclamptic pregnancies were collected immediately after vaginal and abdominal deliveries. Umbilical arteries were isolated and fixed in 10% neutral formaline solution, embedded in paraffin, and then stained with hematoxylin and eosin (H&E) for the histologic investigation, and eNOS activation were examined in samples by streptavidine-biotine immunohistochemical methods. The arterial sections were also fixed in phosphate-buffered 2.5% glutaraldehyde solution (pH 7.2) for 3 h and post-fixed with 1% osmium tetroxide at 4 C for 2 h for the investigation of the ultrastructural examination. In the umbilical artery of preeclamptic pregnancies, endothelial cells were oval, triangular, or polygonal, and were disorganized. Some endothelial cells were separated by enlarged intercellular spaces. A dilated endoplasmic reticulum, swollen mitochondria, and vanished mitochondrial cristae were observed. The nuclei of some endothelial cells displayed deep invaginations and irregular outlines. Most endothelial cells had a high number of cytoplasmic vacuoles. In preeclampsia, eNOS immunoreactivity increased considerably in endothelial cells when compared to normal pregnancies. We believe that preeclampsia plays an important role in the pathogenesis of endothelial cell dysfunction and activation in the umbilical artery. However, the disturbance mechanism of endothelial cells is not known, and further studies are necessary to clarify the exact mechanism.en_US
dc.identifier.doi10.3109/10641961003686468
dc.identifier.endpage463en_US
dc.identifier.issn1064-1963
dc.identifier.issn1525-6006
dc.identifier.issue7en_US
dc.identifier.pmid21029010en_US
dc.identifier.scopus2-s2.0-78049482552en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage458en_US
dc.identifier.urihttps://doi.org/10.3109/10641961003686468
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24491
dc.identifier.volume32en_US
dc.identifier.wosWOS:000283593500009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofClinical And Experimental Hypertensionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectUmbilical Arteryen_US
dc.subjectEnosen_US
dc.subjectLight Microscopyen_US
dc.subjectEndothelial Ultrastructureen_US
dc.subjectPreeclampsiaen_US
dc.subjectResistance Arteriesen_US
dc.subjectWomenen_US
dc.subjectDysfunctionen_US
dc.subjectExpressionen_US
dc.subjectMediatoren_US
dc.titleEndothelial Nitric Oxide Synthase Immunreactivity and the Ultrastructure of Endothelial Cells of Umbilical Artery in Normal and Preeclamptic Pregnanciesen_US
dc.typeArticleen_US

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