High fructose diet suppresses exercise-induced increase in AQP7 expression in the in vivo rat heart

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dc.authoridPalabıyık, Orkide/0000-0002-3488-3740
dc.authoridvardar, selma arzu/0000-0002-1073-1718
dc.authorid, Aziz KARACA/0000-0001-9408-5073
dc.authorwosidPalabıyık, Orkide/Q-2792-2019
dc.contributor.authorKaraca, Aziz
dc.contributor.authorPalabiyik, Orkide
dc.contributor.authorTastekin, Ebru
dc.contributor.authorTuran, Fatma Nesrin
dc.contributor.authorVardar, Selma Arzu
dc.date.accessioned2024-06-12T11:03:19Z
dc.date.available2024-06-12T11:03:19Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractObjective: Cardiac uptake of fructose is thought to be mediated by glucose transporter 5 (GLUT5), whereas the uptake of glycerol is facilitated by aquaporin 7 (AQP7). We aimed to investigate the effect of a high-fructose diet (HFD) on GLUT5 and AQP7 levels in the rat heart subjected to exercise. Methods: Male Sprague-Dawley rats were allocated to control (C; n= 11), exercise (E; n= 10), HFD (n= 12), and HFD plus exercise (HFD-E; n= 12) groups. HFD was started 28 days before euthanasia. From day 24 to 27, rats were subjected to moderate exercise, followed by vigorous exercise on day 28 (groups E and HFD-E). Cardiac GLUT5 and AQP7 mRNA levels were determined using RT-PCR. The protein contents of GLUT5 and AQP7 were immunohistochemically assessed. Paired-t, ANOVA with Bonferroni, Kruskal-Wallis, and Bonferroni-corrected Mann-Whitney U tests were used for statistical analysis. Results: GLUT5 mRNA expression and protein content did not differ between the groups. AQP7 mRNA levels significantly increased (4.8-fold) in group E compared with in group C (p<0.001). Compared with group C, no significant change was observed in AQP7 mRNA levels in groups HFD and HFD-E. The AQP7 staining score in group E was significantly higher than that in groups C (p<0.001), E (p<0.001), and HFD-E (p<0.001). Conclusion: Our study indicates that exercise enhances cardiac AQP7 mRNA expression and protein content. However, HFD prevents the exercise-induced increase in cardiac AQP7 expression. This inhibitory effect may be related to the competition between fructose and glycerol as energy substrates in the rat heart subjected to 5 days of physical exercise.en_US
dc.description.sponsorshipTrakya University Scientific Research Projects Unit [2013/76]en_US
dc.description.sponsorshipThe study was financially supported by the Trakya University Scientific Research Projects Unit (2013/76).en_US
dc.identifier.doi10.14744/AnatolJCardiol.2016.6958
dc.identifier.endpage922en_US
dc.identifier.issn2149-2263
dc.identifier.issn2149-2271
dc.identifier.issue12en_US
dc.identifier.pmid27182614en_US
dc.identifier.scopus2-s2.0-85006355184en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage916en_US
dc.identifier.trdizinid237728en_US
dc.identifier.urihttps://doi.org/10.14744/AnatolJCardiol.2016.6958
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/237728
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21592
dc.identifier.volume16en_US
dc.identifier.wosWOS:000392196800006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Soc Cardiologyen_US
dc.relation.ispartofAnatolian Journal Of Cardiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHigh-Fructose Dieten_US
dc.subjectExerciseen_US
dc.subjectGlucose Transporter 5en_US
dc.subjectAquaporin 7en_US
dc.subjectHearten_US
dc.subjectMetabolic Syndromeen_US
dc.subjectGlycerolen_US
dc.titleHigh fructose diet suppresses exercise-induced increase in AQP7 expression in the in vivo rat hearten_US
dc.typeArticleen_US

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