Investigation of Metallo-Beta-Lactamases in Carbapenem Resistant Pseudomonas aeruginosa Strains by Phenotypic and Genotypic Methods

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dc.contributor.authorAksoy, M. Duygu
dc.contributor.authorTugrul, H. Murat
dc.date.accessioned2024-06-12T11:13:04Z
dc.date.available2024-06-12T11:13:04Z
dc.date.issued2020
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIntroduction: Carbapenem resistant Pseudomonas aeruginosa strains cause serious problems in treatment. A large number of identified metallo-beta-lactamase (MBL) enzymes produced by P. aeruginosa are one of the most important mechanisms in resistance to carbapenems. MBL genes are located on the chromosome or plasmid, and they can easily spread between different bacterial strains. The activities of these enzymes are zinc-dependent, and they are inhibited by ethylenediaminetetraacetic acid (EDTA). Therefore, this advantage is used in MBL identification tests. In this study, it was aimed to determine MBL among P. aeruginosa strains. Materials and Methods: MBL existence was investigated in 35 P. aeruginosa strains accepted to be mildly susceptible/resistant to any of the carbapenem group of antibiotics through phenotypic and genotypic methods. Phenotypic tests were performed as double disk synergy test (DDST), combined disk diffusion tests (CDDT) by using 0.1 M and 0.5 M EDTA, MBL E-test, and modified Hodge test (MHT). bla(IMP), bla(VIM), bla(GIM), bla(SIM), bla(SPM) genes and bla(NDM) gene were investigated by multiplex polimerase chain reaction (PCR) and PCR, respectively. Escherichia coli ATCC 25922 and P. aeruginosa ATCC 27853 standard bacteria were used in tests. VIM-1, VIM-2, IMP-13, SPM-1, NDM-1 type MBL-producing P. aeruginosa strains were used as positive controls. Results: Among the carbapenems resistant P. aeruginosa isolates, positivity of MBL was found as 54.2% by MBL E-test, 42.8% by DDST, 94.2% and 37.1% by CDDT method using 0.5 M and 0.1 M EDTA, respectively. Modified Hodge test and genotypic method did not detect MBL. Conclusion: In order to correctly evaluate the results of the phenotypic method, the investigation of resistance genes by molecular methods is also required. The most common metallo-beta-lactamase enzymes responsible for resistance to carbapenem in Pseudomonas were not observed. It was thought that different mechanisms might be responsible for the identified carbapenem resistance.en_US
dc.identifier.doi10.5578/flora.68921
dc.identifier.endpage307en_US
dc.identifier.issn1300-932X
dc.identifier.issue3en_US
dc.identifier.startpage301en_US
dc.identifier.trdizinid452257en_US
dc.identifier.urihttps://doi.org/10.5578/flora.68921
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/452257
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23401
dc.identifier.volume25en_US
dc.identifier.wosWOS:000582249100003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isotren_US
dc.publisherBilimsel Tip Yayinevien_US
dc.relation.ispartofFlora Infeksiyon Hastaliklari Ve Klinik Mikrobiyoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPseudomonas Aeruginosaen_US
dc.subjectMetallo-Beta-Lactamaseen_US
dc.subjectMultiplex Polymerase Chain Reactionen_US
dc.subjectClinical Isolateen_US
dc.subjectMechanismsen_US
dc.subjectVim-38en_US
dc.subjectOprden_US
dc.titleInvestigation of Metallo-Beta-Lactamases in Carbapenem Resistant Pseudomonas aeruginosa Strains by Phenotypic and Genotypic Methodsen_US
dc.typeArticleen_US

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