Evaluation of the cytotoxic effect of titanium dioxide nanoparticles in human embryonic lung cells

Basit Öğe Kaydı
dc.authoridOltulu, Cagatay/0000-0002-6051-3479
dc.authorwosidOltulu, Cagatay/V-1823-2018
dc.contributor.authorAfsar, Olkan
dc.contributor.authorOltulu, Cagatay
dc.date.accessioned2024-06-12T11:16:27Z
dc.date.available2024-06-12T11:16:27Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground/aim: Titanium dioxide nanoparticles are widely used in a variety of products, including sunscreens, paints, and ceramics. However, their increasing use has raised concerns about their potential health risks. Titanium dioxide nanoparticles have been shown to have the ability to enter the bloodstream and accumulate in various tissues, reaching the fetus via the placenta. The aim of this study was to investigate the cytotoxic effects of titanium dioxide nanoparticles on a human embryonic lung cell line (HEL 299/An1) and the formation of oxidative DNA damage.Materials and methods: The cytotoxic effects of brookite-based titanium dioxide nanoparticles (<100 nm) were assessed using the 3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay for 24 and 48 h. Cell titanium levels were determined using inductively coupled plasma mass spectrometry. Oxidative DNA damage was assessed by measuring the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) as a biomarker.Results: Titanium dioxide nanoparticles caused dose-dependent cytotoxicity in HEL 299/An1 cells. The IC50 values were 25.93 mu M and 0.054 mu M after 24 h and 48 h of exposure, respectively. Cell titanium levels were found to be 25,967 ppb after 24 h and 210,353 ppb after 48 h (p < 0.01). 8-OHdG was detected at 32.96 ng/mL after 24 h of exposure and 17.89 ng/mL after 48 h of exposure.Conclusion: In our study, it was shown that titanium nanoparticles caused dose-dependent cytotoxicity and oxidative DNA damage in human embryonic lung cells. The nanoparticles also accumulated in cells and were taken up in higher amounts after 48 h of exposure. These findings suggest that titanium dioxide nanoparticles may pose a health risk, especially for pregnant women who may not be aware of their pregnancy. Therefore, it is important to take preventive measures to reduce exposure to these nanoparticles.en_US
dc.description.sponsorshipResearch Fund of Trakya University [2021/29]en_US
dc.description.sponsorshipAcknowledgment and/or disclaimers, if any The authors have no conflicts of interest to declare. This work was supported by the Research Fund of Trakya University (Project Number: 2021/29) .en_US
dc.identifier.doi10.55730/1300-0144.5733
dc.identifier.endpage1657en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue6en_US
dc.identifier.pmid38813501en_US
dc.identifier.scopus2-s2.0-85179889684en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1648en_US
dc.identifier.trdizinid1221014en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5733
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1221014
dc.identifier.urihttps://hdl.handle.net/20.500.14551/24322
dc.identifier.volume53en_US
dc.identifier.wosWOS:001127245500027en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTitanium Dioxide Nanoparticlesen_US
dc.subjectOxidative DNA Damageen_US
dc.subjectCytotoxicityen_US
dc.subjectTio2 Nanoparticlesen_US
dc.subjectExposureen_US
dc.subjectDnaen_US
dc.subjectGenotoxicityen_US
dc.subjectParticlesen_US
dc.subjectToxicityen_US
dc.subjectAnataseen_US
dc.subjectPhaseen_US
dc.titleEvaluation of the cytotoxic effect of titanium dioxide nanoparticles in human embryonic lung cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu