Alfa lipoik asidin rat karaciğer homojenatlarında indüklenmiş lipid peroksidasyonuna etkisi
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Dosyalar
Tarih
2006
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Oksidan ürünlerin, diabetes mellitus, ateroskleroz, katarakt ve karaciğer sirozu gibi hastalıkların patogenezinde rol aldığı bilinmektedir. Bu nedenle son yıllarda antioksidan moleküllerin terapotik amaçlı kullanımı artmıştır. Çalışmamızda; alfa lipoik asidin farklı dozlarının invitro kullanımının, rat karaciğer homojenatlarında indüklenmiş lipid peroksidasyonu ve doku glutatyon düzeyine etkilerini araştırmak amaçlanmıştır. Rat karaciğer homojenatlarında hidrojen peroksit (15 mM) ve ferröz amonyum sülfat (2 mM) kullanılarak 2 farklı yöntemle lipid peroksidasyon indüksiyonu yapıldı. Lipid peroksidasyonu indüklenen deney düzenekleri 4 alt gruba ayrıldı ve bu gruplara sırasıyla 0, 2, 4 ve 8 mM alfa lipoik asid eklendi. Doku glutatyon düzeyleri ve lipid peroksidasyon son ürünü olan malondialdehid düzeyleri incelendi. Hem hidrojen peroksit ve hem ferröz amonyum sülfat uygulanan deney düzeneklerinde; aktivasyon gruplarındaki malondialdehid düzeyleri kontrol gruplarına göre anlamlı yüksek bulundu. Alfa lipoik asid uygulanan gruplardaki malondialdehid düzeyleri aktivasyon gruplarındaki düzeylere göre anlamlı düşük bulundu. Aktivasyon gruplarındaki glutatyon düzeyleri kontrol gruplarına göre anlamlı düşük bulundu. Alfa lipoik asid uygulanan gruplardaki glutatyon düzeyleri aktivasyon gruplarındaki düzeylere göre anlamlı yüksek bulundu. Glutatyon düzeyinin zamana bağlı değişimi incelendiğinde önce dereceli olarak azaldığı, daha sonra arttığı, 4 ve 8 mM gruplarında başlangıç düzeyini de aştığı gözlendi. Sonuç olarak bulgularımız, hidrojen peroksit ve ferröz amonyum sülfat uygulanan deney düzeneklerinde lipid peroksidasyonunun uyarıldığını göstermektedir. Alfa lipoik asid uygulanan gruplardaki malondialdehid düzeylerinin aktivasyon gruplarındaki düzeylere göre anlamlı düşük, glutatyon düzeylerinin ise anlamlı yüksek olmasının nedeninin, alfa lipoik asid'in antioksidan özelliklerinden kaynaklandığını söyleyebiliriz.
It is known that oxidant species play a role in the pathogenesis of certain diseases such as diabetes mellitus, atherosclerosis, cataract and hepatic cirrhosis. Therefore, the use of antioxidant species for therapeutic purposes has risen up in the recent years. The aim of the study was to investigate the different concentrations of alpha lipoic acid on the induced lipid peroxidation and tissue glutathione level in rat liver homogenates. Two different methods have been used for lipid peroxidation using hidrogen peroxide (15 mM) and ferrous ammonium sulphate (2 mM). The experimental setups induced by lipid peroxidation have been divided into four sub-groups. Alpha lipoic acid was added in 0, 2, 4 and 8 mM concentrations into those groups, respectively. The malondialdehyde levels which is the end product of lipid peroxidation and the levels of tissue glutathione have been determined. In the experimental setups where both hidrogen peroxide and ferrous ammonium sulphate have been applied, the levels of malondialdehyde in the activation groups have been found to be significantly higher than to the control groups. The levels of malondialdehyde in the groups that alpha lipoic acid has been applied has been found to be significantly lower than the activation groups. The levels of glutathione in the activation groups have been detected significantly lower when it was compared to the control groups. The levels of glutathione in the groups where alpha lipoic acid has been applied has been found to be significantly higher from the activation groups. When the time-dependent change in the level of glutathione was investigated it was observed that this initially decrease and then started to increase. In the groups of 4 and 8 mM, this level was even over from the starting point. In conclusion, our findings suggest that lipid peroxidation is induced in the experimental setups where hidrogen peroxide and ferrous ammonium sulphate are applied. The reason of significantly lower malondialdehyde and higher glutathione levels in alpha lipoic acid group than activation groups may be a result of the antioxidant properity of alpha lipoic acid.
It is known that oxidant species play a role in the pathogenesis of certain diseases such as diabetes mellitus, atherosclerosis, cataract and hepatic cirrhosis. Therefore, the use of antioxidant species for therapeutic purposes has risen up in the recent years. The aim of the study was to investigate the different concentrations of alpha lipoic acid on the induced lipid peroxidation and tissue glutathione level in rat liver homogenates. Two different methods have been used for lipid peroxidation using hidrogen peroxide (15 mM) and ferrous ammonium sulphate (2 mM). The experimental setups induced by lipid peroxidation have been divided into four sub-groups. Alpha lipoic acid was added in 0, 2, 4 and 8 mM concentrations into those groups, respectively. The malondialdehyde levels which is the end product of lipid peroxidation and the levels of tissue glutathione have been determined. In the experimental setups where both hidrogen peroxide and ferrous ammonium sulphate have been applied, the levels of malondialdehyde in the activation groups have been found to be significantly higher than to the control groups. The levels of malondialdehyde in the groups that alpha lipoic acid has been applied has been found to be significantly lower than the activation groups. The levels of glutathione in the activation groups have been detected significantly lower when it was compared to the control groups. The levels of glutathione in the groups where alpha lipoic acid has been applied has been found to be significantly higher from the activation groups. When the time-dependent change in the level of glutathione was investigated it was observed that this initially decrease and then started to increase. In the groups of 4 and 8 mM, this level was even over from the starting point. In conclusion, our findings suggest that lipid peroxidation is induced in the experimental setups where hidrogen peroxide and ferrous ammonium sulphate are applied. The reason of significantly lower malondialdehyde and higher glutathione levels in alpha lipoic acid group than activation groups may be a result of the antioxidant properity of alpha lipoic acid.
Açıklama
Tıpta Uzmanlık Tezi
Anahtar Kelimeler
Alfa Lipoik Asid, Malondialdehid, Glutatyon, Hidrojen Peroksit, Ferröz Amonyum Sülfat
