Eupatilin attenuates TGF-?2-induced proliferation and epithelial-mesenchymal transition of retinal pigment epithelial cells
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose The main characteristic of proliferative vitreoretinopathy (PVR) is migration, adhesion, and epithelial-mesenchymal transition (EMT) of retinal pigment epithelial cells (RPE). Eupatilin is a naturally occurring flavone that has the potential to inhibit cell proliferation and EMT. However, its efficacy on the PVR model induced by transforming growth factor-2 (TGF-beta 2) is unknown. In this study, the potential effect of eupatilin on proliferation and EMT in the treatment of RPE was investigated. Methods Serum starved human RPE cells (ARPE-19) were treated with 10 ng/ml TGF-beta 2 alone or co-treated with 25 mu M eupatilin for 48 h. Quantitative real-time PCR and Western blot analysis were used to assess targets at the mRNA and protein expression level, respectively. Apoptosis and cell cycle progression was assessed by image-based cytometry. The effect of treatment on cell migration was evaluated by wound healing assay. Results Eupatilin inhibited TGF-beta 2-induced RPE cell proliferation via regulating the cell cycle and inducing apoptosis. TGF-beta 2 upregulated mRNA expression of mesenchymal markers fibronectin and vimentin was significantly downregulated by the treatment, while the epithelial markers E-cadherin and occludin expression was upregulated. The therapy significantly suppressed TGF-beta 2 encouraged cell migration through downregulating the expression of transcription factors Twist, Snail, and ZEB1 induced by TGF-beta 2. Furthermore, eupatilin significantly inhibited the expression of MMP-1, -7, and -9, and suppressed NF-kappa B signalling. Conclusion These results suggest that eupatilin could inhibit the proliferation and transformation into fibroblast-like cells of RPE cells; thus the agent may be a potential therapeutic value in treating PVR.
Açıklama
Anahtar Kelimeler
Eupatilin, PVR, Epithelial To Mesenchymal Transition, Retina Pigment Epithelial Cell, TGF-? 2
Kaynak
Cutaneous And Ocular Toxicology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
40
Sayı
2
