Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix

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dc.contributor.authorAktas, G
dc.contributor.authorKayton, R
dc.date.accessioned2024-06-12T11:18:57Z
dc.date.available2024-06-12T11:18:57Z
dc.date.issued2000
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBasic fibroblast growth factor (bFGF) is thought to play an important role in normal tissue repair and wound healing. It is a potent mitogenic and chemotactic factor for fibroblasts, regulating proliferation and extracellular matrix (ECM) production by these cells. In this study, we present morphologic evidence of the ultrastructural location of bFGF in fibroblasts and ECM using several antibodies, tissues, and species. Distinct labeling is seen in the nuclei of fibroblasts and some labeling in the cytosol. Immunolabeling of the cytosol excludes organelles involved in the usual secretory pathway, such as rough endoplasmic reticulum, Golgi apparatus, and secretory vacuoles. The same labeling is observed with either polyclonal or monoclonal antibodies. We suggest that bFGF functions as a nuclear protein in fibroblasts and is not secreted by a normal secretory pathway. Fibroblasts may export bFGF via unique cellular pathways that are clearly distinct from classic signal peptide mediated secretion. This may provide a source for ECM-resident bFGF. The same antibodies show different labeling intensity in the ECM. This protein, through integration into the ECM, may act as a local regulator and promote regeneration of these tissues after wounding. Direct evidence is the dramatic reduction of bFGF labeling in axotomized rat ECM collagen fibers versus control animals.en_US
dc.identifier.endpage233en_US
dc.identifier.issn0301-5564
dc.identifier.issue3en_US
dc.identifier.pmid10817677en_US
dc.identifier.scopus2-s2.0-0034107521en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage227en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14551/25021
dc.identifier.volume113en_US
dc.identifier.wosWOS:000086490400009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Verlagen_US
dc.relation.ispartofHistochemistry And Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGene-Transcriptionen_US
dc.subjectEndothelial-Cellsen_US
dc.subjectLocalizationen_US
dc.subjectReleaseen_US
dc.subjectProduceen_US
dc.subjectBfgfen_US
dc.subjectProteinsen_US
dc.subjectRepairen_US
dc.subjectFamilyen_US
dc.subjectNucleien_US
dc.titleUltrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrixen_US
dc.typeArticleen_US

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