The Effect of a High-Protein Diet and Exercise on Cardiac AQP7 and GLUT4 Gene Expression

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dc.authoridPalabıyık, Orkide/0000-0002-3488-3740
dc.authorid, Aziz KARACA/0000-0001-9408-5073
dc.authoridvardar, selma arzu/0000-0002-1073-1718
dc.authorwosidyamasan, bilge eren/I-8363-2017
dc.authorwosidPalabıyık, Orkide/Q-2792-2019
dc.authorwosidTokuç, Burcu/ABF-7730-2021
dc.contributor.authorPalabiyik, Orkide
dc.contributor.authorKaraca, Aziz
dc.contributor.authorTastekin, Ebru
dc.contributor.authorYamasan, Bilge Eren
dc.contributor.authorTokuc, Burcu
dc.contributor.authorSipahi, Tammam
dc.contributor.authorVardar, Selma Arzu
dc.date.accessioned2024-06-12T10:52:28Z
dc.date.available2024-06-12T10:52:28Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractHigh-protein (HP) diets are commonly consumed by athletes despite their potential health hazard, which is postulated to enforce a negative effect on bone and renal health. However, its effects on heart have not been known yet. Aquaporin-7 (AQP7) is an aquaglyceroporin that facilitates glycerol and water transport. Glycerol is an important cardiac energy production substrate, especially during exercise, in conjunction with fatty acids and glucose. Glucose transporter 4 (GLUT4) is an insulin-sensitive glucose transporter in heart. We aimed to investigate the effect of HPD on AQP7 and GLUT4 levels in the rat heart subjected to exercise. Male Sprague-Dawley rats were divided into control (n = 12), exercise (E) training (n = 10), HPD (n = 12), and HPD-E training (n = 9) groups. The HPD groups were fed a 45 % protein-containing diet 5 weeks. The HPD-E and E groups were performed the treadmill exercise during the 5-week study period. Real-time polymerase chain reaction and immunohistochemistry techniques were used to determine the gene expression and localization of AQP7 and GLUT4 in heart tissue. Results of relative gene expression were calculated by the 'Pfaffl' mathematical method using the REST program. Differences in AQP7 and GLUT4 gene expression were expressed as fold change compared to the control group. Heart weight/tibia ratio and ventricular wall thickness were evaluated as markers of cardiac hypertrophy. Further, serum glucose, glycerol, and insulin levels were also measured. AQP7 gene expression was found to be increased in the E (3.47-fold, p < 0.001), HPD (5.59-fold, p < 0.001), and HPD-E (3.87-fold, p < 0.001) groups compared to the control group. AQP7 protein expression was also increased in the HPD and HPD-E groups (p < 0.001). Additionally, cardiac mRNA expression levels of GLUT4 showed a significant increase in the E (2.16-fold, p < 0.003), HPD (7.14-fold, p < 0.001), and HPD-E (3.43-fold, p < 0.001) groups compared to the control group. GLUT4 protein expression was significantly increased in the E, HPD, and HPD-E groups compared to the control group (p = 0.024, p < 0.001, and p < 0.001, respectively). Furthermore, Serum glucose levels were significantly different between groups (p < 0.005). This difference was observed between the HPD groups and normal-protein diet groups (C and E). Serum insulin levels were higher for HPD groups compared with the normal-protein diet groups (p < 0.001), whereas no differences were observed between the exercise and sedentary groups (p = 0.111). Serum glycerol levels were significantly increased in the HPD groups compared with control and E groups (p < 0.05 and p < 0.05, respectively). Consumption of HPD supplementation caused the increased effects on AQP7 and GLUT4 expression in rat heart.en_US
dc.description.sponsorshipTrakya University, The Faculty of Medicine, Scientific Research Projects Unit (TUBAP) [2013-55]en_US
dc.description.sponsorshipThis study was funded by Trakya University, The Faculty of Medicine, Scientific Research Projects Unit (TUBAP 2013-55).en_US
dc.identifier.doi10.1007/s10528-016-9753-x
dc.identifier.endpage745en_US
dc.identifier.issn0006-2928
dc.identifier.issn1573-4927
dc.identifier.issue5en_US
dc.identifier.pmid27294385en_US
dc.identifier.scopus2-s2.0-84974802660en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage731en_US
dc.identifier.urihttps://doi.org/10.1007/s10528-016-9753-x
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18705
dc.identifier.volume54en_US
dc.identifier.wosWOS:000383038400012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofBiochemical Geneticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAquaporin-7en_US
dc.subjectExerciseen_US
dc.subjectGlucose Transporter 4en_US
dc.subjectGlycerolen_US
dc.subjectHearten_US
dc.subjectHigh-Protein Dieten_US
dc.subjectWhey-Proteinen_US
dc.subjectInsulin-Resistanceen_US
dc.subjectAdipose-Tissueen_US
dc.subjectRatsen_US
dc.subjectGlycerolen_US
dc.subjectSupplementationen_US
dc.subjectSubstrateen_US
dc.subjectAquaglyceroporinsen_US
dc.subjectTranslocationen_US
dc.subjectMetabolismen_US
dc.titleThe Effect of a High-Protein Diet and Exercise on Cardiac AQP7 and GLUT4 Gene Expressionen_US
dc.typeArticleen_US

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