Evaluation of cytological alterations in normal-appearing oral mucosal epithelia of smokers and non-smokers via AgNOR counts and nuclear morphometry
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Dosyalar
Tarih
2008
Dergi Başlığı
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Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sigara içen ve içmeyen kişilerde normal oral mukozaya ait epitel hücrelerindeki proliferatif aktivite AgNOR boyama tekniği ve nükleer morfometri ile değerlendirildi. Hastalar ve Yöntemler: Yaymalar 50-70 yaş arasında sigara içen ve içmeyen, 40’ar hastanın normal görünümlü ağız taban mukozasından elde edildi. İyi tespit edilmiş nükleuslu ilk 50 skuamöz epitel hücresinde AgNOR’lar sayıldı ve bilgisayarlı görüntü analizi ile nükleer alanlar hesaplandı. Bulgular: İstatistiksel olarak sigara içmeyen grupta nukleus başına düşen ortalama AgNOR sayısı (3.47± 0.30) sigara içenlerden daha azdı (4.22±0.39, p<0.001). Ayrıca sigara içenlere ait hücre çekirdeklerinin alan ortalamaları (94.32±10.08) içmeyenlerden daha yüksek bulundu (87±9.4, p<0.05). Beş taneden fazla AgNOR’a sahip olan nukleusların ortalama sayısı sigara içmeyen ve içenlerde sırasıyla %14.6 ve %36.8 olarak bulundu. Sonuç: Bulgularımız sigara içiminin oral proliferatif lezyonların oluşmasında önemli bir risk faktörü olduğunu ve bu lezyonların taranması için oral eksfolyatif sitolojinin tercih edilebilecek bir yöntem olduğunu ortaya koymaktadır.
Objectives: We planned this study to evaluate the proliferative activity of the oral mucosal epithelial cells of smokers and non-smokers via nuclear morphometry and AgNOR counts. Patients and Methods: Smears were collected from normal-appearing mouth floor mucosa of 40 non-smokers and 40 smokers between ages of 50 and 70. AgNORs were counted in the first 50 well-fixed, nucleated squamous cells and nuclear areas were calculated via computerized image analyzing system. Results: Statistically mean AgNOR numbers per nucleus in the nonsmoking group (3.47±0.30) was lower than the smoking group (4.22±0.39, p<0.001), and mean nuclear areas of squamous cells of smokers (94.32±10.08) was also significantly higher than non-smokers (87±9.4, p<0.05). The mean number of nuclei having more than 5 AgNORs was 14.6% and 36.8% in non-smokers and smokers, respectively. Conclusion: Our results support that smoking is a severe risk factor for oral mucosal proliferative lesions and exfoliative cytology can be the preferred method for screening of oral mucosal lesions.
Objectives: We planned this study to evaluate the proliferative activity of the oral mucosal epithelial cells of smokers and non-smokers via nuclear morphometry and AgNOR counts. Patients and Methods: Smears were collected from normal-appearing mouth floor mucosa of 40 non-smokers and 40 smokers between ages of 50 and 70. AgNORs were counted in the first 50 well-fixed, nucleated squamous cells and nuclear areas were calculated via computerized image analyzing system. Results: Statistically mean AgNOR numbers per nucleus in the nonsmoking group (3.47±0.30) was lower than the smoking group (4.22±0.39, p<0.001), and mean nuclear areas of squamous cells of smokers (94.32±10.08) was also significantly higher than non-smokers (87±9.4, p<0.05). The mean number of nuclei having more than 5 AgNORs was 14.6% and 36.8% in non-smokers and smokers, respectively. Conclusion: Our results support that smoking is a severe risk factor for oral mucosal proliferative lesions and exfoliative cytology can be the preferred method for screening of oral mucosal lesions.
Açıklama
Anahtar Kelimeler
Genel ve Dahili Tıp
Kaynak
Trakya Üniversitesi Tıp Fakültesi Dergisi
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
25
Sayı
2
