Anti-inflammatory and antibacterial evaluation of Thymus sipyleus Boiss. subsp sipyleus var. sipyleus essential oil against rhinosinusitis pathogens

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dc.authoridDemirci, Fatih/0000-0003-1497-3017
dc.authoridDemirci, Betul/0000-0003-2343-746X
dc.authorwosidDemirci, Fatih/A-8295-2008
dc.authorwosidkaraca, nursenem/ABA-3107-2020
dc.authorwosidDemirci, Betul/AHA-2298-2022
dc.contributor.authorDemirci, Fatih
dc.contributor.authorKaraca, Nursenem
dc.contributor.authorTekin, Mehmet
dc.contributor.authorDemirci, Betul
dc.date.accessioned2024-06-12T11:13:41Z
dc.date.available2024-06-12T11:13:41Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThymus sipyleus Boiss. subsp. sipyleus var. sipyleus of the Lamiaceae, locally known as thyme scented lemon, which is an endemic taxon collected from Sivas in Anatolia, was investigated in this study due to its folk medicine use against rhinosinusitis. The aromatic characteristics of the plant material gave the idea for the detailed evaluation of the volatiles and essential oil thereof. Consequently, the oil was obtained by Clevenger type hydrodistillation followed by gas chromatography-flame ionization detector (GC-FID) and gas chromato-graphy/mass spectrometry (GC/MS) analyses for phytochemical characterization. To confirm the folk medicinal use against sinusitis, in vitro antimicrobial activities of the essential oil was evaluated by agar diffusion, microdilution and vapour diffusion methods against selected rhinosinusitis associated strains such as Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. epidermidis, Streptococcus pyogenes, S. pneumoniae, Pseudomonas aeruginosa, Haemophilus influenzae and Moraxella catarrhal's. Additionally, the in vitro anti-inflammatory activity was evaluated by 5-lipoxygenase (5-LOX) inhibitory effect of the essential oil spectrophotometrically. Furthermore, the composition of the volatiles of the vapour phase of the oil was determined by headspace-solid phase microextraction (HS-SPME-GC/MS) after 15 min and 24 h in accordance with antimicrobial vapour diffusion method conditions, respectively. According to the analytical results, the main component was determined as thymol (66.2%). Whereas in the HS-SPME method p-cymene (26.1%) and gamma-terpinene (26%) were identified as the main volatile components within the 15. min., and thymol (75.3%) after 24 h, respectively. The antibacterial activity against rhinosinusitis pathogens varied between 160 and 1250 mu g/mL minimum inhibitory concentrations, with the best inhibitory effects observed against the S. aureus, S. pyogenes and M. catarrhalis. The anti-inflammatory activity of the oil was determined as 12.1 +/- 1.8% in 100 mu g/mL. The results showed the in vitro antimicrobial and anti-inflammatory potential of the oil also in vapour phase against sinusitis supporting the traditional use.en_US
dc.description.sponsorshipAnadolu University Scientific Research Projects [BAP 1309S319]en_US
dc.description.sponsorshipThis work was supported by the Anadolu University Scientific Research Projects (BAP 1309S319), which is a part of the MSc work of N. Karaca. Authors declare no conflict of interest.en_US
dc.identifier.doi10.1016/j.micpath.2018.06.025
dc.identifier.endpage121en_US
dc.identifier.issn0882-4010
dc.identifier.pmid29908309en_US
dc.identifier.scopus2-s2.0-85048706497en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage117en_US
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2018.06.025
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23646
dc.identifier.volume122en_US
dc.identifier.wosWOS:000451238000018en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofMicrobial Pathogenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRhinosinusitisen_US
dc.subjectThymus Sipyleusen_US
dc.subjectHS-SPME-GC/MSen_US
dc.subjectAntibacterial Activityen_US
dc.subjectAnti-Inflammatory Activityen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectVapor-Phaseen_US
dc.subjectChemical-Compositionen_US
dc.subjectAntioxidanten_US
dc.subjectConstituentsen_US
dc.subjectCombinationen_US
dc.subjectCinnamonen_US
dc.subjectPlantsen_US
dc.titleAnti-inflammatory and antibacterial evaluation of Thymus sipyleus Boiss. subsp sipyleus var. sipyleus essential oil against rhinosinusitis pathogensen_US
dc.typeArticleen_US

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