A NOVEL BIOSENSOR BASED ON Lactobacillus acidophilus FOR DETERMINATION OF PHENOLIC COMPOUNDS IN MILK PRODUCTS AND WASTEWATER

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dc.authoridPaluzar, Hatice/0000-0001-9232-8748;
dc.authorwosidŞEN, Burhan/AAF-3466-2019
dc.authorwosidPaluzar, Hatice/IZQ-0711-2023
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.authorwosidÖkten, Suzan/HJH-6316-2023
dc.authorwosidOkten, Suzan/HGV-1334-2022
dc.contributor.authorSagiroglu, Ayten
dc.contributor.authorPaluzar, Hatice
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorOkten, Suzan
dc.contributor.authorSen, Burhan
dc.date.accessioned2024-06-12T10:54:41Z
dc.date.available2024-06-12T10:54:41Z
dc.date.issued2011
dc.departmentTrakya Üniversitesien_US
dc.description.abstractDifferent branches of industry need to use phenolic compounds (PCs) in their production, so determination of PCs sensitively, accurately, rapidly, and economically is very important. For the sensitive determination of PCs, some biosensors based on pure polyphenol oxidase, plant tissu, e and microorganisms were developed before. But there has been no study to develop a microbial phenolic compounds biosensor based on Lactobacillus species, which contain polyphenol oxidase enzyme. In this study, we used different forms of Lactobacillus species as enzyme sources of biosensor and compared biosensor performances of these forms for determination of PCs. For this purpose, we used lyophilized Lactobacillus cells (containing L. bulgaricus, L. acidophilus, Streptococcus thermophilus), pure L. acidophilus, pure L. bulgaricus, and L. acidophilus-and L. bulgaricus adapted to catechol in Lactobacilli MRS Broth. The most suitable form was determined and optimization studies of the biosensor were carried out by using this form. For preparing the bioactive layer of the biosensor, the Lactobacillus cells were immobilized in gelatin by using glutaraldehyde. In the study, we used catechol as a substrate. Phenolic compound determination is based on the assay of the differences on the respiration activity of the cells on the oxygen meter in the absence and the presence of catechol. The microbial biosensor response depends directly on catechol concentration between 0.5 and 5.0 mM with 18 min response time. In the optimization studies of the microbial biosensor the most suitable microorganism amount was found to be 10 mg, and also phosphate buffer (pH 8.0; 50 mM) and 37.5 degrees C were obtained as the optimum working conditions. In the characterization studies of the microbial biosensor some parameters such as substrate specificity on the biosensor response and operational and storage stability were examine. Furthermore, the determination of PC levels in synthetic wastewater, industrial wastewater, and milk products was investigated by using the developed biosensor under optimum conditions.en_US
dc.identifier.doi10.1080/10826068.2010.540607
dc.identifier.endpage336en_US
dc.identifier.issn1082-6068
dc.identifier.issue4en_US
dc.identifier.pmid21967334en_US
dc.identifier.scopus2-s2.0-80053650586en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage321en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2010.540607
dc.identifier.urihttps://hdl.handle.net/20.500.14551/19143
dc.identifier.volume41en_US
dc.identifier.wosWOS:000295794600001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectLactobacillius Acidophilusen_US
dc.subjectOxygen Electrodeen_US
dc.subjectPhenolic Compounden_US
dc.subjectProbioticsen_US
dc.subjectMicrobial Biosensoren_US
dc.subjectBisphenol-Aen_US
dc.subjectBacteriaen_US
dc.subjectHumansen_US
dc.titleA NOVEL BIOSENSOR BASED ON Lactobacillus acidophilus FOR DETERMINATION OF PHENOLIC COMPOUNDS IN MILK PRODUCTS AND WASTEWATERen_US
dc.typeArticleen_US

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