Normal diyet ve metiyoninden zengin diyetle beslenen sıçanlarda serum asimetrik dimetil arginin (adma) düzeyleri üzerine vitamin e, vitamin c, vitamin b6 ve folik asidin etkileri
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2008
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info:eu-repo/semantics/openAccess
Özet
Çalışmamızda vitamin E, vitamin C, folik asit ve B6 vitamininin normal diyet ve metiyoninden zengin diyetle beslenen sıçanlarda serum asimetrik dimetil arginin düzeyi üzerine etkilerini saptamaya çalıştık. Çalışmada 2 aylık 100 adet Wistar sıçan her grupta 10 sıçan bulunan 10 gruba ayrıldı. Sıçanlara 5 hafta süreyle normal diyet uygulandı, ilk 5 gruba metiyonin yüklemesi yapılmazken, diğer 5 gruptaki sıçanlara metiyonin yüklemesi (1 g/kg) içme suyuna eklemek suretiyle yapıldı. Ağırlık ölçümleri çalışmanın birinci günü ve dördüncü haftasında yapıldı. Gruplar arasında ağırlık açısından anlamlı bir farklılık gözlemlenmedi. Serum fizyolojik, vitamin E (2 mg/kg/gün), C (250 mg/kg/gün), B6 (5 mg/kg/gün) ve folik asit (4 mg/kg/gün) hem normal hem metiyoninden zengin diyet uygulanan gruplara bir hafta süreyle intraperitonal olarak uygulandı. Oral metiyonin yüklemesi plazma homosistein düzeylerini artırırken (%70,3; p<0.05), plazma ADMA, SDMA ve L-arginin düzeylerini etkilemedi. Metiyonin yüklemesinin yaptığı plazma homosistein düzeyi artışı folik asit (%45,9; p<0,05), vitamin B6 (%34,0; p<0,05) ve vitamin C (%34,3; p<0,05) ile istatistiksel anlamlı derecede azaldı. Buna karşılık vitamin E plazma homosistein düzeyleri üzerine anlamlı bir etki oluşturmadı. Sonuç olarak; oral metiyonin yüklemesi plazma homosistein düzeylerinde bir artışa yol açarken, plazma ADMA, SDMA ve L-arginin düzeylerini etkilememektedir. Metiyonin yüklemesinin yaptığı plazma homosistein düzeyi artışını folik asit, vitamin B6 ve vitamin C azaltmakta, vitamin E etkilemektedir. Anahtar kelimeler: Asimetrikdimetil arginin, homosistein, metiyonin, vitamin E, vitamin C, vitamin B6, folik asit, sıçan
In our study we tried to establish the effects of vitamin E, vitamin C, vitamin B6 and folic acid on plasma asymmetric dimethylarginine levels in rats fed by normal and methionine-rich diet. In the study, 2 month old 100 Wistar rat were divided into 10 groups of 10 rats each. Rats were fed normal diet for 5 weeks; the first five groups of rats omitted methionine, the other 5 groups of rats excess methionine by adding (1 g/kg) to drinking water. Weights were measured in the first day and 4th week of the study. No significant differences were observed between the groups in weight measurements. Normal saline, vitamin E (2 mg/kg/day), C (250 mg/kg/day), B6 (5 mg/kg/day) and folic acid (4 mg/kg/day) were administered intraperitoneally to normal and methionine-rich diet group for one week. While oral methionine loading was increased plasma levels of homocysteine (%70,3; p<0.05) it has no effect in the levels of plasma ADMA, SDMA and L-arginine. Elevation of plasma homocysteine levels which was induced by methionine loading was significantly decreased with vitamin B6 (34%; p<0,05), vitamin C (34.3%; p<0,05) and folic acid (45,9%; p<0,05). On the other hand, vitamin E has no significantly effect in the levels of homocysteine. In conclusion; oral administration of methionine loading had no efffect in the levels of plasma ADMA, SDMA and L-arginine while it caused an increase in the levels of homocysteine. Folic acid, vitamin B6, vitamin C were decreased elevation of plasma homocysteine levels which was induced by methionine loading, but vitamin E has no effect. Key words: Asymmetric dimethylarginine, homocystein, methionine, vitamin E, vitamin C, vitamin B6, folic acid, rat
In our study we tried to establish the effects of vitamin E, vitamin C, vitamin B6 and folic acid on plasma asymmetric dimethylarginine levels in rats fed by normal and methionine-rich diet. In the study, 2 month old 100 Wistar rat were divided into 10 groups of 10 rats each. Rats were fed normal diet for 5 weeks; the first five groups of rats omitted methionine, the other 5 groups of rats excess methionine by adding (1 g/kg) to drinking water. Weights were measured in the first day and 4th week of the study. No significant differences were observed between the groups in weight measurements. Normal saline, vitamin E (2 mg/kg/day), C (250 mg/kg/day), B6 (5 mg/kg/day) and folic acid (4 mg/kg/day) were administered intraperitoneally to normal and methionine-rich diet group for one week. While oral methionine loading was increased plasma levels of homocysteine (%70,3; p<0.05) it has no effect in the levels of plasma ADMA, SDMA and L-arginine. Elevation of plasma homocysteine levels which was induced by methionine loading was significantly decreased with vitamin B6 (34%; p<0,05), vitamin C (34.3%; p<0,05) and folic acid (45,9%; p<0,05). On the other hand, vitamin E has no significantly effect in the levels of homocysteine. In conclusion; oral administration of methionine loading had no efffect in the levels of plasma ADMA, SDMA and L-arginine while it caused an increase in the levels of homocysteine. Folic acid, vitamin B6, vitamin C were decreased elevation of plasma homocysteine levels which was induced by methionine loading, but vitamin E has no effect. Key words: Asymmetric dimethylarginine, homocystein, methionine, vitamin E, vitamin C, vitamin B6, folic acid, rat
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Anahtar Kelimeler
Eczacılık ve Farmakoloji, Pharmacy and Pharmacology
