IDENTIFICATION AND PRODUCTION OF PHENOLIC NICOTIFLORIN IN ASTRAGALUS CHRYSOCHLORUS CALLUS

Basit Öğe Kaydı
dc.authoridÇakır, Özgür/0000-0003-3075-683X
dc.authoridKara, Neslihan Turgut/0000-0001-5355-4937
dc.authoridkarabey, fatih/0000-0003-0305-6192
dc.authorwosidARI, SULE/B-4143-2018
dc.authorwosidHASANÇEBİ, Semra/AAO-4409-2021
dc.authorwosidÇakır, Özgür/A-8998-2018
dc.authorwosidÇakır, Özgür/AAX-1485-2021
dc.authorwosidKara, Neslihan Turgut/B-4846-2015
dc.contributor.authorTurgut-Kara, Neslihan
dc.contributor.authorCakir, Ozgur
dc.contributor.authorHasancebi, Semra
dc.contributor.authorKarabey, Fatih
dc.contributor.authorAri, Sule
dc.date.accessioned2024-06-12T10:52:44Z
dc.date.available2024-06-12T10:52:44Z
dc.date.issued2018
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAstragalus chrysochlorus Boiss. & Kotschy (2n = 16) is one of the rare Turkish endemic species and it is listed in the Red Data Book of Turkish Plants as endangered. This species has been used traditionally for its wound healing properties and a crude ethanol extract prepared from the roots exhibits antioxidant and cytotoxic activities. In this study, a detailed phytochemical analysis was performed on A. chrysochlorus calli that resulted in the isolation of a major constituent. The purified molecule's structure elucidation was completed by spectral methods [nuclear magnetic resonance (NMR) and mass spectrometry (MS)], which revealed a rarely encountered the flavonoid in the Astragalus genus, nicotiflorin. In order to increasing nicotiflorin content in the callus cultures, the effects of culturing time and elicitor treatment were investigated. The HPLC analyses showed that the maximal production of nicotiflorin occurred with long-term cultured (13 years old) callus as 4775 mu g/g dry weight (DW), whereas it was 132 mu g/g DW for short-term cultured (2 months old) ones. Then, the 24 h treatment of the yeast extract that was used as biotic elicitor had negative effect on the production of nicotiflorin. The data obtained from this study could be significant for the mass production of nicotiflorin from long term in vitro cultured A. chrysochlorus callus.en_US
dc.description.sponsorshipResearch Fund of the Istanbul University [40151]en_US
dc.description.sponsorshipThis study was financially supported by the Research Fund of the Istanbul University (Project numbers 40151). We thank Prof. Dr. Erdal Bedir for comments that greatly improved the manuscript.en_US
dc.identifier.doi10.31925/farmacia.2018.3.25
dc.identifier.endpage562en_US
dc.identifier.issn0014-8237
dc.identifier.issn2065-0019
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85049487824en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage558en_US
dc.identifier.urihttps://doi.org/10.31925/farmacia.2018.3.25
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18801
dc.identifier.volume66en_US
dc.identifier.wosWOS:000437399100025en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSoc Stiinte Farmaceutice Romaniaen_US
dc.relation.ispartofFarmaciaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAstragalusen_US
dc.subjectNicotiflorinen_US
dc.subjectKaempferol 3-O-Rutinosideen_US
dc.subjectFlavonoiden_US
dc.subjectNMRen_US
dc.subjectHPLCen_US
dc.subjectSecondary Metabolismen_US
dc.subjectFlavonol Glycosidesen_US
dc.subjectOxidative Stressen_US
dc.subjectCell-Culturesen_US
dc.subjectResponsesen_US
dc.subjectExtracten_US
dc.titleIDENTIFICATION AND PRODUCTION OF PHENOLIC NICOTIFLORIN IN ASTRAGALUS CHRYSOCHLORUS CALLUSen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu