Effects of Organophosphorus and Pyrethroid pesticides on antioxidant enzymes and reactivation effects of Pralidoxime:In vitro studies

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dc.authoridPaluzar, Hatice/0000-0001-9232-8748
dc.authorwosidPaluzar, Hatice/IZQ-0711-2023
dc.contributor.authorPaluzarl, Hatice
dc.contributor.authorSagiroglu, Ayten
dc.date.accessioned2024-06-12T10:59:14Z
dc.date.available2024-06-12T10:59:14Z
dc.date.issued2022
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe present study aimed to assess the inhibition effects of organophosphate pesticides, malathion(R), dichlorvos(R); pyrethroid pesticides, deltamethrin(R), lambda-cyhaloethrin(R) on antioxidant enzymes and the reactivation ability of pralidoxime against pesticide inhibited-antioxidant enzymes. Oximes were reported by reactivation ability against organophosphate inhibited-acetylcholinesterase and we focused to investigate the reactivation effect of pralidoxime against organophosphate inhibited-antioxidant enzymes. IC50 values were determined by means of activity percentage diagrams. The concentrations of deltamethrin(R), malathion(R), dichlorvos(R), lambda-cyhaloethrin(R) that inhibited 50% of catalase were 5.2 mu M, 158 mu M, 133 mu M, 320 mu M, respectively, inhibited 50% of superoxide dismutase were 62 mu M, 240 mu M, 328 mu M, 2320 mu M, respectively and inhibited 50% of glutathione peroxidase were 0.7 mu M, 1198 mu M, 1638 mu M, 98 mu M, respectively. All pesticide doses showed an inhibition effect on antioxidant enzymes. Deltamethrin(R) wasfound to be a more potent inhibitor for the antioxidant enzymes followed by the rest of the pesticides used in this study. The reactivation effect of pralidoxime was determined for organophosphate inhibited-enzymes. Reactivation results showed that only catalase is reactivated by pralidoxime against dichlorvos(R) and malathion(R). Under the exposure of 50-800 mu M malathion(R) concentrations, the activities of catalase were calculated as 72-11%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 92-31% and 98-39%, respectively. Under the exposure of 100-1500 mu M dichlorvos(R) concentrations, the activities of catalase were calculated as 50-6%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 95-30% and 93-28%, respectively. When the results are examined, it is seen that increasing the pralidoxime concentration does not significantly affect the reactivation percentage of the catalase enzyme.en_US
dc.description.sponsorshipTrakya University Research Fund [TUBAP 2012/122]; Trakya University Research Funden_US
dc.description.sponsorshipThe present study was financially supported by Trakya University Research Fund (Project No: TUBAP 2012/122). We are grateful to Trakya University Research Fund for its support.en_US
dc.identifier.doi10.48129/kjs.11847
dc.identifier.issn2307-4108
dc.identifier.issn2307-4116
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85136681194en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.48129/kjs.11847
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20375
dc.identifier.volume49en_US
dc.identifier.wosWOS:000810360300007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcademic Publication Councilen_US
dc.relation.ispartofKuwait Journal Of Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDeltamethrinen_US
dc.subjectDichlorvosen_US
dc.subjectLambda-Cyhaloethrinen_US
dc.subjectMalathionen_US
dc.subjectPralidoximeen_US
dc.subjectSuperoxide-Dismutaseen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectHuman Exposureen_US
dc.subjectChlorpyrifosen_US
dc.subjectErythrocytesen_US
dc.subjectDeterminantsen_US
dc.subjectInsecticidesen_US
dc.subjectRisken_US
dc.titleEffects of Organophosphorus and Pyrethroid pesticides on antioxidant enzymes and reactivation effects of Pralidoxime:In vitro studiesen_US
dc.typeArticleen_US

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