Effects of Organophosphorus and Pyrethroid pesticides on antioxidant enzymes and reactivation effects of Pralidoxime:In vitro studies
dc.authorid | Paluzar, Hatice/0000-0001-9232-8748 | |
dc.authorwosid | Paluzar, Hatice/IZQ-0711-2023 | |
dc.contributor.author | Paluzarl, Hatice | |
dc.contributor.author | Sagiroglu, Ayten | |
dc.date.accessioned | 2024-06-12T10:59:14Z | |
dc.date.available | 2024-06-12T10:59:14Z | |
dc.date.issued | 2022 | |
dc.department | Trakya Üniversitesi | en_US |
dc.description.abstract | The present study aimed to assess the inhibition effects of organophosphate pesticides, malathion(R), dichlorvos(R); pyrethroid pesticides, deltamethrin(R), lambda-cyhaloethrin(R) on antioxidant enzymes and the reactivation ability of pralidoxime against pesticide inhibited-antioxidant enzymes. Oximes were reported by reactivation ability against organophosphate inhibited-acetylcholinesterase and we focused to investigate the reactivation effect of pralidoxime against organophosphate inhibited-antioxidant enzymes. IC50 values were determined by means of activity percentage diagrams. The concentrations of deltamethrin(R), malathion(R), dichlorvos(R), lambda-cyhaloethrin(R) that inhibited 50% of catalase were 5.2 mu M, 158 mu M, 133 mu M, 320 mu M, respectively, inhibited 50% of superoxide dismutase were 62 mu M, 240 mu M, 328 mu M, 2320 mu M, respectively and inhibited 50% of glutathione peroxidase were 0.7 mu M, 1198 mu M, 1638 mu M, 98 mu M, respectively. All pesticide doses showed an inhibition effect on antioxidant enzymes. Deltamethrin(R) wasfound to be a more potent inhibitor for the antioxidant enzymes followed by the rest of the pesticides used in this study. The reactivation effect of pralidoxime was determined for organophosphate inhibited-enzymes. Reactivation results showed that only catalase is reactivated by pralidoxime against dichlorvos(R) and malathion(R). Under the exposure of 50-800 mu M malathion(R) concentrations, the activities of catalase were calculated as 72-11%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 92-31% and 98-39%, respectively. Under the exposure of 100-1500 mu M dichlorvos(R) concentrations, the activities of catalase were calculated as 50-6%, respectively. After, inhibited catalase was incubated with 1 mM and 10 mM pralidoxime, the activities of catalase were calculated as 95-30% and 93-28%, respectively. When the results are examined, it is seen that increasing the pralidoxime concentration does not significantly affect the reactivation percentage of the catalase enzyme. | en_US |
dc.description.sponsorship | Trakya University Research Fund [TUBAP 2012/122]; Trakya University Research Fund | en_US |
dc.description.sponsorship | The present study was financially supported by Trakya University Research Fund (Project No: TUBAP 2012/122). We are grateful to Trakya University Research Fund for its support. | en_US |
dc.identifier.doi | 10.48129/kjs.11847 | |
dc.identifier.issn | 2307-4108 | |
dc.identifier.issn | 2307-4116 | |
dc.identifier.issue | 3 | en_US |
dc.identifier.scopus | 2-s2.0-85136681194 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.uri | https://doi.org/10.48129/kjs.11847 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14551/20375 | |
dc.identifier.volume | 49 | en_US |
dc.identifier.wos | WOS:000810360300007 | en_US |
dc.identifier.wosquality | Q3 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.language.iso | en | en_US |
dc.publisher | Academic Publication Council | en_US |
dc.relation.ispartof | Kuwait Journal Of Science | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Deltamethrin | en_US |
dc.subject | Dichlorvos | en_US |
dc.subject | Lambda-Cyhaloethrin | en_US |
dc.subject | Malathion | en_US |
dc.subject | Pralidoxime | en_US |
dc.subject | Superoxide-Dismutase | en_US |
dc.subject | Lipid-Peroxidation | en_US |
dc.subject | Human Exposure | en_US |
dc.subject | Chlorpyrifos | en_US |
dc.subject | Erythrocytes | en_US |
dc.subject | Determinants | en_US |
dc.subject | Insecticides | en_US |
dc.subject | Risk | en_US |
dc.title | Effects of Organophosphorus and Pyrethroid pesticides on antioxidant enzymes and reactivation effects of Pralidoxime:In vitro studies | en_US |
dc.type | Article | en_US |