Matrix metalloproteinase 9 (MMP9) expression in preeclamptic decidua and MMP9 induction by tumor necrosis factor alpha and interleukin 1 beta in human first trimester decidual cells

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dc.authoridUz, Yesim/0000-0002-0381-4590
dc.contributor.authorLockwood, Charles J.
dc.contributor.authorOner, Ceyda
dc.contributor.authorUz, Yesim H.
dc.contributor.authorKayisli, Umit A.
dc.contributor.authorHuang, S. Joseph
dc.contributor.authorBuchwalder, Lynn F.
dc.contributor.authorMurk, William
dc.date.accessioned2024-06-12T10:59:07Z
dc.date.available2024-06-12T10:59:07Z
dc.date.issued2008
dc.departmentTrakya Üniversitesien_US
dc.description.abstractExtravillous trophoblasts (EVTs) invade human decidua via sequential integrin-mediated binding and proteolysis of basement membrane proteins in the extracellular matrix (ECM). In preeclampsia, shallow EVT invasion impairs spiral artery and arteriole remodeling to reduce uteroplacental blood flow. Excess decidual cell-expressed matrix metalloproteinases (MMPs) 2 and 9, in response to preeclampsia-related interleukin 1 beta (IL1B) and tumor necrosis factor alpha (TNF), may inappropriately degrade these basement membrane proteins and impede EVT invasion. This study found significantly higher immunohistochemical MMP9 levels in decidual cells and adjacent interstitial trophoblasts in placental sections of preeclamptic versus gestational age-matched control women. In contrast, immunostaining for MMP2 and tissue inhibitor of matrix metalloproteinases 1 and 2 (TIMP1 and TIMP2) were similar in preeclamptic and control groups. First-trimester decidual cells were incubated with estradiol (E-2) or E-2 + medroxyprogesterone acetate (MPA), with or without TNF or IL1B. As measured by ELISA, both cytokines elicited concentration-dependent increases in secreted MMP9 levels that were unaffected by MPA. In contrast, secreted levels of MMP2, TIMP1, and TIMP2 were unchanged in all treatment groups. Substrate gel zymography and Western blotting confirmed that each cytokine increased secreted levels of MMP9 but not MMP2. Similarly, quantitative RT-PCR found that TNF and IL1B enhanced MMP9, but not MMP2, mRNA levels. At the implantation site, inflammatory cytokine-enhanced MMP9 may promote preeclampsia by disrupting the decidual ECM to interfere with normal stepwise EVT invasion.en_US
dc.description.sponsorshipNHLBI NIH HHS [1 R01 HL070004-04, R01 HL070004-02, R01 HL070004-01A1, R01 HL070004, R01 HL070004-04, R01 HL070004-03] Funding Source: Medline; NICHD NIH HHS [2 R01HD33937-05, R01 HD033937, R01 HD033937-05] Funding Source: Medlineen_US
dc.identifier.doi10.1095/biolreprod.107.063743
dc.identifier.endpage1072en_US
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.issue6en_US
dc.identifier.pmid18276934en_US
dc.identifier.scopus2-s2.0-44349095791en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1064en_US
dc.identifier.urihttps://doi.org/10.1095/biolreprod.107.063743
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20324
dc.identifier.volume78en_US
dc.identifier.wosWOS:000256056800013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Press Incen_US
dc.relation.ispartofBiology Of Reproductionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCytokinesen_US
dc.subjectDeciduaen_US
dc.subjectExtracellular Matrixen_US
dc.subjectMatrix Metalloproteinaseen_US
dc.subjectPregnancyen_US
dc.subjectMatrix-Metalloproteinase Expressionen_US
dc.subjectHuman Extravillous Trophoblasten_US
dc.subjectGrowth-Factor-Betaen_US
dc.subjectTissue Inhibitorsen_US
dc.subject1st Trimesteren_US
dc.subjectIn-Vitroen_US
dc.subjectExtracellular-Matrixen_US
dc.subjectMediated Migrationen_US
dc.subjectHuman Endometriumen_US
dc.subjectTnf-Alphaen_US
dc.titleMatrix metalloproteinase 9 (MMP9) expression in preeclamptic decidua and MMP9 induction by tumor necrosis factor alpha and interleukin 1 beta in human first trimester decidual cellsen_US
dc.typeArticleen_US

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