Development of callus colonies from the isolated microspore culture of capsicum annuum l.

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dc.authorscopusid23097104100
dc.authorscopusid6602972194
dc.authorscopusid6602653719
dc.authorscopusid15019145900
dc.contributor.authorBal U.
dc.contributor.authorAbak K.
dc.contributor.authorBüyükalaca S.
dc.contributor.authorComlekcioglu N.
dc.date.accessioned2024-06-12T10:25:14Z
dc.date.available2024-06-12T10:25:14Z
dc.date.issued2003
dc.description.abstractAndrogenesis in Capsicum annuum L. via isolated microspore culture, with a limited scope, was studied. Donor genotype, i.e. U-130, was a doubled haploid. Intact flower buds containing microspores from early to late uninucleate stages were pretreated with a mannitol starvation medium both at 10 and 35°C for 7 days. High temperature treatment tested here, although considered effective in Capsicum anther culture, was detrimental and therefore flower buds from this treatment were not used. Following pretreatment at 10°C microspores were isolated and cultured on NLN medium (20) without growth regulators. Osmoticum and carbon was supplied to the microspores with 100, 130, and 170 g/1 sucrose. Only 170 g/1 culture was maintained and the rest of the cultures were lost to contamination. Callus colonies were obtained on the 30th and 60th days at rate of 40.5 and 67%, respectively. Callus colonies were subcultured to MS media containing 2, 4-D at 1 and 2 mg/1, but further development was not obtained. It was concluded that the approach adopted earlier on the cellular and molecular characterization studies of induced multinucleate Capsicum microspores (12, 14, 15) may lead to high frequency regeneration of haploids via callus colonies, as obtained in the present study, hence, isolated microspore culture using mannitol starvation as pretreatment has potentiality in Capsicum annum L. breeding. © 2003 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/13102818.2003.10817056
dc.identifier.endpage43en_US
dc.identifier.issn1310-2818
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-1242284532en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage38en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2003.10817056
dc.identifier.urihttps://hdl.handle.net/20.500.14551/16256
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbon; Mannitol; Sucrose; Androgenesis; Article; Bud; Callus Culture; Cell Nucleus; Contamination; Controlled Study; Culture Medium; Doubled Haploid Line; Flower; Genotype; Growth Regulation; Haploidy; High Temperature Procedures; Isolation Procedure; Microspore Culture; Multinuclear Cell; Nonhuman; Pepper; Plant Breeding; Plant Growth; Capsicum; Capsicum Annuumen_US
dc.titleDevelopment of callus colonies from the isolated microspore culture of capsicum annuum l.en_US
dc.typeArticleen_US

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