N-acetylcysteine counteracts oxidative stress and protects alveolar epithelial cells from lung contusion-induced apoptosis in rats with blunt chest trauma

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dc.authoridAkpolat Ferah, Meryem/0000-0002-3419-1728
dc.authoridCerkezkayabekir, Aysegul/0000-0001-5537-1042
dc.authoridtarladacalisir, taner/0000-0003-0499-341X
dc.authoridkaramustafaoglu, yekta altemur/0000-0002-5491-1219
dc.authoridUz, Yesim/0000-0002-0381-4590
dc.authorwosidAkpolat Ferah, Meryem/ABD-3035-2020
dc.authorwosidCerkezkayabekir, Aysegul/Q-6629-2018
dc.authorwosidtarladacalisir, taner/HGE-7061-2022
dc.authorwosidkaramustafaoglu, yekta altemur/S-9512-2019
dc.contributor.authorTopcu-Tarladacalisir, Yeter
dc.contributor.authorTarladacalisir, Taner
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorKaramustafaoglu, Altemur
dc.contributor.authorUz, Yesim Hulya
dc.contributor.authorAkpolat, Meryem
dc.contributor.authorCerkezkayabekir, Aysegul
dc.date.accessioned2024-06-12T11:07:55Z
dc.date.available2024-06-12T11:07:55Z
dc.date.issued2014
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the protective effects of N-acetylcysteine (NAC) on peroxidative and apoptotic changes in the contused lungs of rats following blunt chest trauma. The rats were randomly divided into three groups: control, contusion, and contusion + NAC. All the rats, apart from those in the control group, performed moderate lung contusion. A daily intramuscular NAC injection (150 mg/kg) was given immediately following the blunt chest trauma and was continued for two additional days following cessation of the trauma. Samples of lung tissue were taken in order to evaluate the tissue malondialdehyde (MDA) level, histopathology, and epithelial cell apoptosis using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and active caspase-3 immunostaining. In addition, we immunohistochemically evaluated the expression of surfactant protein D (SP-D) in the lung tissue. The blunt chest trauma-induced lung contusion resulted in severe histopathological injury, as well as an increase in the MDA level and in the number of cells identified on TUNEL assay together with active caspase-3 positive epithelial cells, but a decrease in the number of SP-D positive alveolar type 2 (AT-2) cells. NAC treatment effectively attenuated histopathologic, peroxidative, and apoptotic changes, as well as reducing alterations in SP-D expression in the lung tissue. These findings indicate that the beneficial effects of NAC administrated following blunt chest trauma is related to the regulation of oxidative stress and apoptosis.en_US
dc.description.sponsorshipTrakya University Research Center, Edirne, Turkey [2012- 90]en_US
dc.description.sponsorshipThis study is supported by Trakya University Research Center, Edirne, Turkey, financially (Project No: 2012- 90).en_US
dc.identifier.doi10.1007/s10735-014-9563-6
dc.identifier.endpage471en_US
dc.identifier.issn1567-2379
dc.identifier.issn1567-2387
dc.identifier.issue4en_US
dc.identifier.pmid24442604en_US
dc.identifier.scopus2-s2.0-84904322072en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage463en_US
dc.identifier.urihttps://doi.org/10.1007/s10735-014-9563-6
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22234
dc.identifier.volume45en_US
dc.identifier.wosWOS:000338284700011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Molecular Histologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLung Contusionen_US
dc.subjectApoptosisen_US
dc.subjectOxidative Stressen_US
dc.subjectN-Acetylcysteineen_US
dc.subjectRaten_US
dc.subjectAcetyl-L-Cysteineen_US
dc.subjectPulmonary Contusionen_US
dc.subjectSurfactant Dysfunctionen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectHydrogen-Peroxideen_US
dc.subjectBarrier Functionen_US
dc.subjectInjuryen_US
dc.subjectModelen_US
dc.subjectAciden_US
dc.subjectResolutionen_US
dc.titleN-acetylcysteine counteracts oxidative stress and protects alveolar epithelial cells from lung contusion-induced apoptosis in rats with blunt chest traumaen_US
dc.typeArticleen_US

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