Detection of Parathyroid Hormone Using an Electrochemical Impedance Biosensor Based on PAMAM Dendrimers

Basit Öğe Kaydı
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.authorwosidÖZCAN, Hakkı/AAJ-6857-2021
dc.contributor.authorOzcan, Hakki Mevlut
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2024-06-12T11:12:04Z
dc.date.available2024-06-12T11:12:04Z
dc.date.issued2015
dc.departmentTrakya Üniversitesien_US
dc.description.abstractThis paper presents a novel hormone-based impedimetric biosensor to determine parathyroid hormone (PTH) level in serum for diagnosis and monitoring treatment of hyperparathyroidism, hypoparathyroidism and thyroid cancer. The interaction between PTH and the biosensor was investigated by an electrochemical method. The biosensor was based on the gold electrode modified by 12-mercapto dodecanoic (12MDDA). Antiparathyroid hormone (anti-PTH) was covalently immobilized on to poly amidoamine dendrimer (PAMAM) which was bound to a 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) couple, self-assembled monolayer structure from one of the other NH2 sites. The immobilization of anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscope techniques. After the optimization studies of immobilization materials such as 12MDDA, EDC-NHS, PAMAM, and glutaraldehyde, the performance of the biosensor was investigated in terms of linearity, sensitivity, repeatability, and reproducibility. PTH was detected within a linear range of 10-60 fg/mL. Finally the described biosensor was used to monitor PTH levels in artificial serum samples. (C) 2015 American Institute of Chemical Engineersen_US
dc.description.sponsorshipTrakya University Scientific Research Foundation [TUBAP-2001/195]; TUBITAK (The Scientific and Technological Research Council of Turkey) [113 Z 678]en_US
dc.description.sponsorshipSupport from Trakya University Scientific Research Foundation (Project Number: TUBAP-2001/195) and TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 113 Z 678) are gratefully acknowledged.en_US
dc.identifier.doi10.1002/btpr.2060
dc.identifier.endpage822en_US
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.issue3en_US
dc.identifier.pmid25683333en_US
dc.identifier.scopus2-s2.0-84931575696en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage815en_US
dc.identifier.urihttps://doi.org/10.1002/btpr.2060
dc.identifier.urihttps://hdl.handle.net/20.500.14551/23034
dc.identifier.volume31en_US
dc.identifier.wosWOS:000356670700025en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology Progressen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectParathyroid Hormoneen_US
dc.subjectBiosensoren_US
dc.subjectPAMAMen_US
dc.subjectElectrochemical Impedance Spectroscopyen_US
dc.subject12-Mercapto Dodecanoicen_US
dc.subjectPrimary Hyperparathyroidismen_US
dc.subjectMultilayer Filmsen_US
dc.subjectGlandsen_US
dc.subjectImmobilizationen_US
dc.subjectSpectroscopyen_US
dc.subjectImmunoassayen_US
dc.subjectCalciumen_US
dc.subjectSensorsen_US
dc.titleDetection of Parathyroid Hormone Using an Electrochemical Impedance Biosensor Based on PAMAM Dendrimersen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu