Proteome profiling of the compatible interaction between wheat and stripe rust

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dc.authoridinan, cihan/0000-0003-0288-5917
dc.authoridKorkut, Senay Vural/0000-0002-6260-0357
dc.authoridGÃœNEL, ASLIHAN/0000-0001-5301-2628
dc.authoridARDA, NAZLI/0000-0002-1043-5652
dc.authoridGunel, Aslihan/0000-0001-5301-2628
dc.authoridBaykal, Ahmet Tarik/0000-0002-8814-7351
dc.authoridDemirci, Yahya Emin/0000-0003-4065-3498
dc.authorwosidinan, cihan/AAT-5624-2020
dc.authorwosidKorkut, Senay Vural/ABA-2454-2020
dc.authorwosidGÃœNEL, ASLIHAN/AAE-4361-2019
dc.authorwosidARDA, NAZLI/A-9033-2018
dc.authorwosidGunel, Aslihan/AAP-3816-2021
dc.authorwosidHASANÇEBİ, Semra/AAO-4409-2021
dc.authorwosidBaykal, Ahmet Tarik/C-3948-2013
dc.contributor.authorDemirci, Yahya Emin
dc.contributor.authorInan, Cihan
dc.contributor.authorGunel, Aslihan
dc.contributor.authorMaytalman, Dilara
dc.contributor.authorMert, Zafer
dc.contributor.authorBaykal, A. Tarik
dc.contributor.authorKorkut, Senay Vural
dc.date.accessioned2024-06-12T10:59:40Z
dc.date.available2024-06-12T10:59:40Z
dc.date.issued2016
dc.departmentTrakya Üniversitesien_US
dc.description.abstractOver the last decade, comparative molecular profiling studies between compatible and incompatible plant-pathogen interactions have shown that susceptible response of the host to a pathogen requires factors that promote disease development. In this study, we examined proteome profiles during a compatible interaction between wheat and stripe rust. A 2D-LC system (ProteomeLab PF2D) was used for protein separation and to compare the proteome from infected and control samples. More than 700 protein peaks at each time point were compared between pathogen- and mock-inoculated samples. Selected proteins, with significant differences in abundance were identified by nanoLC-ESI- MS/MS and generated spectra were searched against the wheat protein databases from UniProt, and NCBI and the Puccinia database from The Broad Institute. In total, the identified proteins comprised of 62 % wheat and 38 % Pst proteins. All identified proteins were searched by bioinformatics-based algorithms to detect their subcellular localization and signal peptide motifs which have the potential to catch the candidate effector proteins. The wheat proteins were classified based on their function. Although a compatible interaction, many wheat proteins, such as antioxidants, PRs and cold-responsive proteins, are implicated in defense and stress tolerance. On the pathogen side, 64 proteins were identified, and included some important pathogenicity proteins that can play role in pathogen virulence and suppress the host defense. In addition, we discovered that nine proteins have a signal sequence and three of the hypothetical fungal proteins, PGTG_11681T0, PGTG_07231T0 and CBH50687.1, have been tentatively identified as candidate effectors.en_US
dc.description.sponsorshipTUBITAK [109 T293]en_US
dc.description.sponsorshipThis study was supported by TUBITAK, COST programme 109 T293 project. We thank Kadir Akan, Aysc Yildiz and Lutfi Cetin for their help during plant inoculation and sampling. We also thank Abdulmecit Gokce and Yavuz Ozturk for their technical support for PF2D as well as Konca Bulut and Rahmi Buyukkeskin for their experimental assistance.en_US
dc.identifier.doi10.1007/s10658-016-0882-1
dc.identifier.endpage962en_US
dc.identifier.issn0929-1873
dc.identifier.issn1573-8469
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84957657848en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage941en_US
dc.identifier.urihttps://doi.org/10.1007/s10658-016-0882-1
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20530
dc.identifier.volume145en_US
dc.identifier.wosWOS:000379860300018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Journal Of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDiseaseen_US
dc.subjectEffectoren_US
dc.subjectFungal Proteinsen_US
dc.subjectWheaten_US
dc.subjectPlant-Pathogen Interactionen_US
dc.subjectPuccinia Striiformisen_US
dc.subjectPathogenesis-Related Proteinsen_US
dc.subjectLipid Transfer Proteinen_US
dc.subjectGene-Expressionen_US
dc.subjectIncompatible Interactionsen_US
dc.subjectAntifungal Activityen_US
dc.subjectDisease Resistanceen_US
dc.subjectTriticum-Aestivumen_US
dc.subjectOxidative Stressen_US
dc.subjectHexaploid Wheaten_US
dc.subjectDefenseen_US
dc.titleProteome profiling of the compatible interaction between wheat and stripe rusten_US
dc.typeArticleen_US

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