Development of effective AmB/AmB-?CD complex double loaded liposomes using a factorial design for systemic fungal infection treatment

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dc.authoridYılmaz, Şükran/0000-0002-7945-1124
dc.authoridMutlu-Agardan, N. Basaran/0000-0002-4882-3124
dc.authorwosidYılmaz, Şükran/ABZ-6448-2022
dc.authorwosidONURDAG, Fatma KAYNAK/T-2518-2017
dc.authorwosidAgardan, N. Basaran Mutlu/AEI-9890-2022
dc.authorwosidCELEBİ, Nevin/AAL-6931-2021
dc.contributor.authorMutlu-Agardan, N. Basaran
dc.contributor.authorYilmaz, Sukran
dc.contributor.authorKaynak Onurdag, Fatma
dc.contributor.authorCelebi, Nevin
dc.date.accessioned2024-06-12T11:07:41Z
dc.date.available2024-06-12T11:07:41Z
dc.date.issued2021
dc.departmentTrakya Üniversitesien_US
dc.description.abstractAmphotericin B (AmB) is a very potent antibiotic which still remains as the gold standard for the treatment of systemic fungal infections. AmB is a member of Biopharmaceutical Classification System Class IV, mainly characterized by its poor solubility and low permeability. In this study, AmB/AmB-alpha cyclodextrin complex double loaded liposomes (DLLs) were developed using the design of experiments (DoE (R)) approach to optimize/determine the effects of lipid composition and other parameters on final product properties such as encapsulation efficacy, particle size, polydispersity index, and zeta potential. Experimental design 2(4) was used for optimization of these properties in which four factors were studied in two levels. DLLs showed much higher physical stability than liposomes loaded only with free AmB by the means of particle size, zeta potential and encapsulation efficiency, in addition exhibited sustained release of AmB over 72 h (26.7%) with faster onset time. On the other hand, fourfold improved antimicrobial efficiency, minimum inhibitory concentration (0.125 mu g/ml), and minimum fungicidal concentration (0.5 mu g/ml) was determined by DLLs against C. albicans compared to Ambisome. Dose dependent effects of the DLLs were investigated by cytotoxicity studies on Vero and L-929 cells. No significant cytotoxicity observed for AmB/AmB-alpha CD complex DLLs and Ambisome at tested concentrations while free AmB caused severe cytotoxicity. Lastly the developed DLLs did not cause an increase in NGAL (an early biomarker for acute kidney toxicity) levels for both Vero and HK-2 cell lines compared to free AmB.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [116S047]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under Grant number 116S047.en_US
dc.identifier.doi10.1080/08982104.2020.1755980
dc.identifier.endpage188en_US
dc.identifier.issn0898-2104
dc.identifier.issn1532-2394
dc.identifier.issue2en_US
dc.identifier.pmid32290745en_US
dc.identifier.scopus2-s2.0-85085708722en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage177en_US
dc.identifier.urihttps://doi.org/10.1080/08982104.2020.1755980
dc.identifier.urihttps://hdl.handle.net/20.500.14551/22147
dc.identifier.volume31en_US
dc.identifier.wosWOS:000538320700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal Of Liposome Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLiposomesen_US
dc.subjectCyclodextrinsen_US
dc.subjectAmphotericin Ben_US
dc.subjectDouble Loaded Liposomesen_US
dc.subjectAmphotericin-Ben_US
dc.subjectIn-Vitroen_US
dc.subjectDelivery-Systemsen_US
dc.subjectNanoparticlesen_US
dc.subjectFormulationen_US
dc.subjectAntifungalen_US
dc.subjectReleaseen_US
dc.subjectDrugsen_US
dc.subjectBioavailabilityen_US
dc.subjectNanosuspensionsen_US
dc.titleDevelopment of effective AmB/AmB-?CD complex double loaded liposomes using a factorial design for systemic fungal infection treatmenten_US
dc.typeArticleen_US

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