Protective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileum

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dc.authoridOzacmak, V. Haktan/0000-0003-2651-8353
dc.authoridAktas, Ranan/0000-0002-4474-7371
dc.authoridArslan, Seyfullah Oktay/0000-0001-9328-9373
dc.authoridArslan, Seyfullah Oktay/0000-0001-9328-9373
dc.authorwosidOzacmak, V. Haktan/J-2395-2019
dc.authorwosidAktas, Ranan/F-2910-2016
dc.authorwosidArslan, Seyfullah Oktay/AAE-9538-2019
dc.authorwosidArslan, Seyfullah Oktay/IXN-3383-2023
dc.contributor.authorOzacmak, VH
dc.contributor.authorSayan, H
dc.contributor.authorArslan, SO
dc.contributor.authorAltaner, S
dc.contributor.authorAktas, RG
dc.date.accessioned2024-06-12T10:58:24Z
dc.date.available2024-06-12T10:58:24Z
dc.date.issued2005
dc.departmentTrakya Üniversitesien_US
dc.description.abstractFree radicals derived from molecular oxygen have been reported to be responsible for changes in motility and mucosal damage observed in intestinal ischemia-reperfusion injury. Melatonin has been considered as an antioxidant that prevents injuries resulted from I/R in various tissues. The present study was designed to determine the effect of melatonin on the contractile responses of acetylcholine (Ach) and KCl, on malondialdehyde (MDA), a product of lipid peroxidation, and reduced glutathione (GSH) levels and to assess histopathological changes in the smooth muscle of terminal ileum subjected to ischemia-reperfusion. The intestinal ischemia-reperfusion was induced by occlusion of superior mesenteric artery of rat for 30 min, followed by a period of reperfusion for 3 h. Melatonin at doses of 10 or 50 mg/kg was administered via the tail vein in 5 min prior to reperfusion. Following reperfusion, segments of terminal ileum were rapidly taken and transferred into isolated organ bath and responses to Ach and KCl were recorded. Samples of terminal ileum were also taken for measuring the MDA and GSH levels. EC50 values of these contracting substances were seriously reduced in the ischemia-reperfusion group compared to that of the sham-operated control group. The decreased contraction response to Ach and KCl was significantly ameliorated by a dosage of 50 mg/kg of melatonin, while not by a dosage of 10 mg/kg. Similar pattern of the effect was observed in the tissue levels of MDA and GSH as well as in histological improvement. Melatonin appeared to be restoring the amounts of tissue MDA and GSH back to about control levels. These results suggest that the high dose of melatonin not only physiologically but also biochemically and morphologically could be useful to normalize contractility injured by oxidative stress in intestinal ischemia-reperfusion. (C) 2004 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.lfs.2004.08.031
dc.identifier.endpage1588en_US
dc.identifier.issn0024-3205
dc.identifier.issn1879-0631
dc.identifier.issue14en_US
dc.identifier.pmid15680167en_US
dc.identifier.scopus2-s2.0-12844269268en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1575en_US
dc.identifier.urihttps://doi.org/10.1016/j.lfs.2004.08.031
dc.identifier.urihttps://hdl.handle.net/20.500.14551/20057
dc.identifier.volume76en_US
dc.identifier.wosWOS:000226848100003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofLife Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectContractile Responseen_US
dc.subjectIleumen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectMelatoninen_US
dc.subjectRaten_US
dc.subjectNitric-Oxide Synthaseen_US
dc.subjectTransient Forebrain Ischemiaen_US
dc.subjectSplanchnic Artery-Occlusionen_US
dc.subjectIntestinal Motilityen_US
dc.subjectIschemia/Reperfusion Injuryen_US
dc.subjectLipid-Peroxidationen_US
dc.subjectModelen_US
dc.subjectPreventionen_US
dc.subjectDamageen_US
dc.titleProtective effect of melatonin on contractile activity and oxidative injury induced by ischemia and reperfusion of rat ileumen_US
dc.typeArticleen_US

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