Optimization of ?-amylase immobilization in calcium alginate beads

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dc.authoridErtan, Figen/0000-0002-7882-3993
dc.contributor.authorErtan, Figen
dc.contributor.authorYagar, Hulya
dc.contributor.authorBalkan, Bilal
dc.date.accessioned2024-06-12T10:51:19Z
dc.date.available2024-06-12T10:51:19Z
dc.date.issued2007
dc.departmentTrakya Üniversitesien_US
dc.description.abstractalpha-Amylase enzyme was produced by Aspergillus sclerotiorum under SSF conditions, and immobilized in calcium alginate beads. Effects of immobilization conditions, such as alginate concentration, CaCl2 concentration, amount of loading enzyme, bead size, and amount of beads, on enzymatic activity were investigated. Optimum alginate and CaCl2 concentration were found to be 3% (w/v). Using a loading enzyme concentration of 140 U mL(-1), and bead (diameter 3 mm) amount of 0.5 g, maximum enzyme activity was observed. Beads prepared at optimum immobilization conditions were suitable for up to 7 repeated uses, losing only 35% of their initial activity. Among the various starches tested, the highest enzyme activity (96.2%) was determined in soluble potato starch hydrolysis for 120 min at 40 degrees C.en_US
dc.identifier.doi10.1080/10826060701386679
dc.identifier.endpage204en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue3en_US
dc.identifier.pmid17516249en_US
dc.identifier.scopus2-s2.0-34249052181en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage195en_US
dc.identifier.urihttps://doi.org/10.1080/10826060701386679
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18310
dc.identifier.volume37en_US
dc.identifier.wosWOS:000247309800003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectAlginateen_US
dc.subjectEntrapmenten_US
dc.subjectAspergillus Sclerotiorumen_US
dc.subjectSSFen_US
dc.subjectStarchen_US
dc.subjectHydrolysisen_US
dc.subjectHydrolysisen_US
dc.subjectStarchen_US
dc.titleOptimization of ?-amylase immobilization in calcium alginate beadsen_US
dc.typeArticleen_US

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