Yüksek doz radyoaktif iyot tedavisine bağlı tükürük bezi hasarının önlenmesinde Amifostin, L-Karnitin ve E Vitaminin radyoprotektif etkisinin karşılaştırılması
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Dosyalar
Tarih
2009
Yazarlar
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Yayıncı
Trakya Üniversitesi Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada yüksek doz radyoaktif iyot tedavisine bağlı tükürük bezi hasarına kaşı amifostin, L-karnitin ve E vitaminin radyoprotektif etkisi sintigrafik ve histopatolojik olarak karşılaştırılması amaçlandı. Çaışmada 40 erişkin kobay çaışmaya dahil edildi. 20 kobaya tiroit ablasyonu ve I-131 ile radyoaktif iyot tedavisi tükürük bezi hasarı oluşturmak üzere ip yoldan 30 mCi/kg I-131 uygulandı. Denekler 8 gruba ayrıldı: (1) Grup 1: 200 mg/kg, ip, amifostin radyoaktif iyot tedavisi uygulamasından 30 dakika önce uygulandı ; (2) Grup 2: Radyoaktif iyot tedavisi ve 200 mg/kg, i.p., L-karnitin 10 gün süre ile uygulandı; (3) Grup 3: radyoaktif iyot tedavisi ve 40 mg/kg, im, E vitamini 10 gün süre ile uygulandı (4) Grup 4: 200 mg/kg: ip, amifostine ip serum fizyolojikden 30 dakika önce uygulandı; (5) Grup 5: ve 200 mg/kg i.p., L-karnitin 10 gün süre ile uygulandı, (6) Grup 6: 40 mg/kg, im, E vitamini 10 gün süre ile uygulandı, (7) Grup 7: 30mg/kg I-131 ip uyguland ve (8) Grup 8: Sağlıklı kontrol grubu. Tedaviden 1 ay sonra tükürük bezi sintigrafisi çekildi. Tükürük bezi hasarının derecesi ışık mikroskobu ve histopatolojik skorlama ile değerlendirildi. Tedavi sonrası gruplar arasında vücut ağırlığı ve tiroid fonksiyonları açısından anlamlı fark izlendi. Radyoaktif iyot tedavisi uygulandığındanda radyoaktif iyotun submandibular bezi parotise göre daha fazla etkiledii ve hasara uğrattığı saptandı. Amifostin, L-karnitin ve E-vitamininden her birinin ayrı ayrı kullanımlarının radyoaktif iyot tedavisine balğlı hasardan tükürük bezini farklı düzeylerde koruduğu, ancak hiçbir ajanın mutlak koruma sağlamadığı izlendi.
Abstract
The aim of the present study was to compare the protective effects of amifostine, L-carnitine and vitamin E against high dose radioactive iodine threatment induced salivary gland damage using salivary glan scintigraphy and histopathological examination. 131 I i.p. Forty adult guinea pigs were studied. Twenty guinea pigs received 30 mCi/kg to ablate the thyroid and significantly impair parenchymal function of the salivary glands. Subjects were divided into eight groups as follows: (1) Group 1: 200 mg/kg, i.p., amifostine 30 min prior to radioactive iodine threatment; (2) Group 2: 200 mg/kg, i.p., L-carnitine during ten days; (3) Group 3:40 mg/kg, im, E vitamine during ten days (4) Group 4: 200 mg/kg, ip, amifostine 30 min prior to sham radioactive iodine threatment; (5) Group 5: 200 mg/kg, i.p., L-carnitine during ten days, (6) Group 6:40 mg/kg, im, E vitamine during ten days; (7) Group 131 I, i.p., for modelining radioactive iodine threatment; and (8) Group 8: 1 mL/kg, 7: 30 mCi/kg ip, normal saline for modelling sham radioactive iodine threatment. Scintigraphy was performed 1 months after treatment. Salivary glands were examined by light microscopy and a histopathological scoring system was used to assess the degree of salivary gland damage. There were no no statistically significant differences body weight and thyroid hormon level between the groups after treatment. Radioactive iodine threatment was effected in submandibular gland more than parotid gland. Amifostin, L-carnitine and vitamin E in each of them separately, use-dependent damage of radioactive iodine threatment preserve different levels of salivary gland, but any agent does not provide absolute protection.
Abstract
The aim of the present study was to compare the protective effects of amifostine, L-carnitine and vitamin E against high dose radioactive iodine threatment induced salivary gland damage using salivary glan scintigraphy and histopathological examination. 131 I i.p. Forty adult guinea pigs were studied. Twenty guinea pigs received 30 mCi/kg to ablate the thyroid and significantly impair parenchymal function of the salivary glands. Subjects were divided into eight groups as follows: (1) Group 1: 200 mg/kg, i.p., amifostine 30 min prior to radioactive iodine threatment; (2) Group 2: 200 mg/kg, i.p., L-carnitine during ten days; (3) Group 3:40 mg/kg, im, E vitamine during ten days (4) Group 4: 200 mg/kg, ip, amifostine 30 min prior to sham radioactive iodine threatment; (5) Group 5: 200 mg/kg, i.p., L-carnitine during ten days, (6) Group 6:40 mg/kg, im, E vitamine during ten days; (7) Group 131 I, i.p., for modelining radioactive iodine threatment; and (8) Group 8: 1 mL/kg, 7: 30 mCi/kg ip, normal saline for modelling sham radioactive iodine threatment. Scintigraphy was performed 1 months after treatment. Salivary glands were examined by light microscopy and a histopathological scoring system was used to assess the degree of salivary gland damage. There were no no statistically significant differences body weight and thyroid hormon level between the groups after treatment. Radioactive iodine threatment was effected in submandibular gland more than parotid gland. Amifostin, L-carnitine and vitamin E in each of them separately, use-dependent damage of radioactive iodine threatment preserve different levels of salivary gland, but any agent does not provide absolute protection.
Açıklama
Tıpta Uzmanlık Tezi
Anahtar Kelimeler
Radyoaktif İyot, Amifostin, L-karnitin, E Vitamini, Tükürük Bezi, Radioactive Iodine Threatment, Salivary Gland, Amifostine, L-carnitine, Vitamin E
