Cytological assessments and a methodological approach to observe the apoptotic effect of nickel sulfate (NiSO4) on Allium cepa L. root germination using EB/AO fluorescence staining

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dc.authorscopusid57202917733
dc.authorscopusid58987472900
dc.authorscopusid58986942100
dc.authorscopusid58986767700
dc.contributor.authorGökalp F.D.
dc.contributor.authorGüzey D.
dc.contributor.authorAbis M.
dc.contributor.authorMert Ö.
dc.date.accessioned2024-06-12T10:26:36Z
dc.date.available2024-06-12T10:26:36Z
dc.date.issued2024
dc.description.abstractEthidium bromide–acridine orange (EB/AO) is one of the fastest, most economically valid methods for separating living and dead cells in plant root tips. The present study aimed to investigate the apoptotic effect, nuclear abnormalities and cell division index using an Allium cepa test assay with EB/AO staining and an ImageJ program. Nickel sulphate (NiSO4) concentrations (1.75, 3.5, 7 and 14 ppm) were exposed to the root germination of A. cepa for 48 and 72 h to observe mitotic abnormalities, cytotoxic effects and 5-day exposure to apoptotic effects. It was found that amorphous nuclei, vacuolisation and C-mitosis were the most observed abnormalities, which increased at 14 ppm NiSO4 exposure after 48 and 72 h. The total number of nuclear abnormalities significantly increased at all concentrations and exposure periods. The ImageJ program was used to determine apoptosis rates. The data obtained showed that high concentrations of NiSO4 caused significantly more cell death in root tips than in the control group, resulting in root growth inhibition and increased apoptosis with increased concentration and exposure period. NiSO4 caused toxic activity on root growth, identified as apoptosis, especially at the cortex and vascular region in the root tips. The study found affected damaged and apoptotic areas on the root tips using an EB/AO staining method, which can be used as a marker of damaged tissue areas. © 2024, Horticulture and Forestry Society from Transylvania. All rights reserved.en_US
dc.identifier.doi10.55779/nsb16111676
dc.identifier.issn2067-3264
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85190425474en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.urihttps://doi.org/10.55779/nsb16111676
dc.identifier.urihttps://hdl.handle.net/20.500.14551/16925
dc.identifier.volume16en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherHorticulture and Forestry Society from Transylvaniaen_US
dc.relation.ispartofNotulae Scientia Biologicaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcridine Orange; Allium Cepa; Apoptosis; Ethidyum Bromide; Nickel; Nuclear Abnormalitiesen_US
dc.titleCytological assessments and a methodological approach to observe the apoptotic effect of nickel sulfate (NiSO4) on Allium cepa L. root germination using EB/AO fluorescence stainingen_US
dc.typeArticleen_US

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