Solid state fermentation for the production of ?-amylase from Penicillium chrysogenum using mixed agricultural by-products as substrate
Küçük Resim Yok
Tarih
2006
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Production of a-amylase from local isolate, Penicillium chrysogenum, under solid-state fermentation (SSF) was carried out in this study. Different agricultural by-products, such as wheat bran (WB), sunflower oil meal (SOM), and sugar beet oil cake (SBOC), were used as individual substrate for the enzyme production. WB showed the highest enzyme activity (750 U/gds). Combination of WB, SOM, and SBOC (1:3:1 w/w/w) resulted in a higher enzyme yield (845 U/gds) in comparison with the use of the individual substrate. This combination was used as mixed solid substrate for the production of a-amylase from P. chrysogenum by SSF. Fermentation conditions were optimized. Maximum enzyme yield (891 U/gds) was obtained when SSF was carried out using WB + SOM + SBOC (1:3:1 w/w/w), having initial moisture of 75 %, inoculum level of 20 %, incubation period of 7 days at 30 degrees C. Galactose (1 % w/w), urea and peptone (1 % w/w), as additives, caused increase in the enzyme activity.
Açıklama
Anahtar Kelimeler
Alpha-Amylase, Penicillium Chrysogenum, Solid-State Fermentation, Wheat Bran, Sunflower Oil Meal, Sugar Beet Oil Cake, Aspergillus-Oryzae, Starch, Biosynthesis, Glucoamylase, Hydrolysis, Culture, Waste, Niger
Kaynak
Biologia
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
61
Sayı
6
