A new ITO-based A?42 biosensor for early detection of Alzheimer's disease

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dc.contributor.authorAltay, Dilek Nur
dc.contributor.authorYagar, Hulya
dc.contributor.authorOzcan, Hakki Mevlut
dc.date.accessioned2024-06-12T10:51:35Z
dc.date.available2024-06-12T10:51:35Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractIn this study, a novel label-free impedimetric immunosensor was fabricated for rapid, selective, and sensitive quantitative analysis of A & beta;42 protein for use in the diagnosis of Alzheimer's disease. The immunosensor was fabricated using inexpensive and disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrodes. After the electrodes were modified with 3-glycidoxypropyldimethoxymethylsilane (GPDMMS), the antibody specific to the A & beta;42 protein (anti-A & beta;42) was immobilized. The affinity interaction between anti-A & beta;42 and A & beta;42 in the immobilization steps in immunosensor fabrication and in the quantitation of A & beta;42 were analyzed using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) techniques. Additionally, the morphological changes occurring on the electrode surface during each immobilization step were imaged using scanning electron microscopy (SEM). The linear detection range of the immunosensor was determined as 1-100 pg/mL with the limit of detection value of 0.37 pg/mL. Analytical properties of the biosensor, including reproducibility, repeatability, storage stability, selectivity, and regeneration were investigated. The kinetic behavior of antibody-antigen complex formation was determined for the first time using single frequency impedance (SFI) analysis on an A & beta;42 biosensor. The potential for use of the immunosensor in clinical studies was demonstrated by analysis of A & beta;42 in commercially purchased human serum.en_US
dc.description.sponsorshipScientific Research Projects Unit of Trakya University [TUBAP 2019/167]en_US
dc.description.sponsorshipThis research project was funded by the Scientific Research Projects Unit of Trakya University, (Project number: TUBAP 2019/167) .en_US
dc.identifier.doi10.1016/j.bioelechem.2023.108501
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid37421689en_US
dc.identifier.scopus2-s2.0-85165234880en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2023.108501
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18390
dc.identifier.volume153en_US
dc.identifier.wosWOS:001036856600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmyloid-Beta 1-42 Peptideen_US
dc.subjectAlzheimer's Disease Diagnosisen_US
dc.subjectElectrochemical Biosensoren_US
dc.subjectImmunosensoren_US
dc.subjectImpedimetric Biosensoren_US
dc.subjectElectrochemical Immunosensoren_US
dc.subjectBiomarker Detectionen_US
dc.subjectPolymeren_US
dc.subjectSystemen_US
dc.titleA new ITO-based A?42 biosensor for early detection of Alzheimer's diseaseen_US
dc.typeArticleen_US

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