Effect of chronic physiological stress on rat oocyte reserve: Role of IGF-1, AMH and Bcl-2

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dc.authoridErcetin, Deniz/0000-0001-9713-044X
dc.contributor.authorErcetin, Deniz
dc.contributor.authorSapmaz-Metin, Melike
dc.contributor.authorTopuz, Ruhan Deniz
dc.date.accessioned2024-06-12T10:52:11Z
dc.date.available2024-06-12T10:52:11Z
dc.date.issued2023
dc.departmentTrakya Üniversitesien_US
dc.description.abstractOvarian activity has a complex physiology and is related to oocyte quality in women. This study investigates the effects of ovarian activity with chronic stress (CS) on behavioural parameters, estrous cycles, and ovarian follicular development in rats. Here, we examined the ovarian microenvironment against exposure to stress and to elucidate the stress-related ovarian molecular mechanisms. Twenty female Sprague-Dawley rats were divided into the control and the CS groups. The estrous cycle phases were detected by vaginal smear. Rats in the CS group were immobilized 1 h/day for 8 weeks. At the end of the experiment, all animals were subjected to behavioral tests in their metestrus phase and sacrificed on the other day (diestrus phase). The ovaries were harvested for histological analysis, blood samples were taken to measure cortisol levels. The immunoreactivities of ovarian IGF-1, AMH and Bcl-2 proteins were evaluated by immunohistochemistry. Here, we have studied CS-induced prolonged estrous cycles. We showed a lower number of developing follicles but the higher number of atretic follicles in the CS group's ovaries. The dominant structure of ovarian histology was large interstitial glands. CS caused decreases in ovarian Bcl-2, IGF-1 and AMH immunoreactivities which have roles in follicular development. Also, anxiety was detected in CS-exposed animals. Our results showed that chronic restrainer stress can be a serious endocrine disrupter by reducing ovarian paracrine factors.en_US
dc.description.sponsorshipTrakya University Research Center, Edirne, Turkey [2016-79]en_US
dc.description.sponsorshipThe authors acknowledge Trakya University Research Center, Edirne, Turkey for the financial support (project no: 2016-79) .en_US
dc.identifier.doi10.56042/ijeb.v61i06.1929
dc.identifier.endpage402en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issue6en_US
dc.identifier.startpage393en_US
dc.identifier.urihttps://doi.org/10.56042/ijeb.v61i06.1929
dc.identifier.urihttps://hdl.handle.net/20.500.14551/18621
dc.identifier.volume61en_US
dc.identifier.wosWOS:001021604600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal Of Experimental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnxietyen_US
dc.subjectChronic Stressen_US
dc.subjectEstrous Cycleen_US
dc.subjectFollicular Developmenten_US
dc.subjectOvaryen_US
dc.subjectChronic Restraint Stressen_US
dc.subjectSex-Differencesen_US
dc.subjectBehavioren_US
dc.subjectCycleen_US
dc.subjectExpressionen_US
dc.subjectDepressionen_US
dc.subjectHormonesen_US
dc.subjectLevelen_US
dc.subjectOvaryen_US
dc.titleEffect of chronic physiological stress on rat oocyte reserve: Role of IGF-1, AMH and Bcl-2en_US
dc.typeArticleen_US

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