The effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in rats

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dc.authoridUzun, Hafize/0000-0002-1347-8498
dc.authorwosidUzun, Hafize/D-4811-2019
dc.contributor.authorAyvaz, Suleyman
dc.contributor.authorKanter, Mehmet
dc.contributor.authorAksu, Burhan
dc.contributor.authorSahin, Sevtap Hekimoglu
dc.contributor.authorUzun, Hafize
dc.contributor.authorErboga, Mustafa
dc.contributor.authorPul, Mehmet
dc.date.accessioned2024-06-12T11:02:55Z
dc.date.available2024-06-12T11:02:55Z
dc.date.issued2013
dc.departmentTrakya Üniversitesien_US
dc.description.abstractBackground: The aim of this study was to evaluate the preventive and therapeutic potential of hyperbaric oxygen therapy (HBO) on the liver tissue against bile duct ligation (BDL)-induced oxidative damage and fibrosis in rats. Materials and methods: We divided 32 adult male Sprague Dawley rats into four groups: sham, sham plus HBO, BDL, and BDL plus HBO; each group contained eight animals. We placed the sham plus HBO and BDL plus HBO groups in an experimental hyperbaric chamber in which we administered pure oxygen at 2.5 atmospheres absolute 100% oxygen for 90 min on 14 consecutive days. Results: The application of BDL clearly increased the tissue malondialdehyde level, myeloperoxidase activity, and hydroxyproline content and decreased the antioxidant enzymes (superoxide dismutase and catalase activities) and glutathione level. Hyperbaric oxygen therapy treatment significantly decreased the elevated tissue malondialdehyde level, myeloperoxidase activity, and hydroxyproline content and increased the reduced superoxide dismutase and catalase activities and glutathione level in the tissues. The changes demonstrating the bile duct proliferation and fibrosis in expanded portal tracts include the extension of proliferated bile ducts into lobules, mononuclear cells, and neutrophil infiltration into the widened portal areas were observed in BDL group. Treatment of BDL with HBO attenuated alterations in liver histology. Alpha smooth muscle actin, cytokeratinpositive ductular proliferation, and the activity of terminal deoxynucleotidyl transferase 2'-deoxyuridine, 5'-triphosphate nick end labeling in the BDL decreased with HBO treatment. Conclusions: The data indicate that HBO attenuates BDL-induced oxidative injury, hepatocytes damage, bile duct proliferation, and fibrosis. The hepatoprotective effect of HBO is associated with antioxidative potential. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipTrakya University Research Center, Edirne, Turkey [2011-196]en_US
dc.description.sponsorshipThis study was supported under Project TUBAB 2011-196 by the Trakya University Research Center, Edirne, Turkey.en_US
dc.identifier.doi10.1016/j.jss.2012.12.036
dc.identifier.endpage155en_US
dc.identifier.issn0022-4804
dc.identifier.issn1095-8673
dc.identifier.issue1en_US
dc.identifier.pmid23462455en_US
dc.identifier.scopus2-s2.0-84879075178en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage146en_US
dc.identifier.urihttps://doi.org/10.1016/j.jss.2012.12.036
dc.identifier.urihttps://hdl.handle.net/20.500.14551/21472
dc.identifier.volume183en_US
dc.identifier.wosWOS:000320599600026en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofJournal Of Surgical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHBOen_US
dc.subjectOxidative Stressen_US
dc.subjectImmunohistochemistryen_US
dc.subjectTUNELen_US
dc.subjectBile Duct Ligationen_US
dc.subjectBiliary-Obstructed Ratsen_US
dc.subjectLiver Fibrosisen_US
dc.subjectMelatoninen_US
dc.subjectReperfusionen_US
dc.subjectApoptosisen_US
dc.subjectInjuryen_US
dc.subjectCellsen_US
dc.subjectMechanismsen_US
dc.subjectExpressionen_US
dc.subjectQuercetinen_US
dc.titleThe effects of hyperbaric oxygen application against cholestatic oxidative stress and hepatic damage after bile duct ligation in ratsen_US
dc.typeArticleen_US

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